Molecular Epidemiology Of Respiratory Syncytial Virus In Children In Wuhan Area

Respiratory syncytial virus (RSV) infection in infants is very common with respiratory pathogens, especially infants between 2 to 6 months. The RSV infection often causes severe pneumonia and bronchiolitis, and it usually occurs in the late winter and spring. Respiratory syncytial virus infection is common in young children, in particular, occur in that less than six months, and is far more serious and repeated infections. The RSV infections are also found in older adults, even all ages.Respiratory infections are fairly common virus pathogens.108 specimens of respiratory infected children gathered from Tongji Hospital, Wuhan Children Hospital and Hanyang Hospital were tested and genotyped based on nucleotide sequence by the method of multiple RT-PCR and Real-Time PCR. Parts of specimens were used for virus isolation, using Hep-2 cell as the sentitive cell.2 of the isolated strains were choosen for G gene cloning, sequencing and phylogenetic analysis to reveal the molecular epidemiology of RSV infection in children in Wuhan area. For more information about the fuction of G gene encodind protein and its proteomics research, we constructed the cell model which including recombinant plasmid pEGFP-N1-hRSV. The results showed that there were 23 RSV positive cases of specimens among 108 specimens,16 of RSV A,7 of RSV B. The molecular epidemiology research about the G gene of 2 RSV showed that both of them belonged to subtype A of RSV, sharing high homology. We successful constructed the recombinant RSV G gene transfected Hela cell model, which could provide an effective means and methods for the further research of the G gene function and its proteomics.