XRCC1Gene Polymorphisms And Susceptibility To S And Esophageal Cancer In Ningxia Hui And Han Population

Objective To investigate X-ray repair cross complementing gene1Arg194Trp Arg280His Arg399Gln polymorphism distribution of the Han andHui people in Ningxia esophageal cancer（EC） and normal populations and toanalyze XRCC1polymorphism and its association with susceptibility to EC in Huiand Han people then compare with their difference,Simultaneously analyze therelationship between the environmental factors and esophageal cancer.Methods Collect the whole blood specimens of88patients with EC inNingxia， among the group38Hui cases and50Han cases while in the controlgroup of100patients,(Hui50cases and Han50cases). Hui people must be threegenerations to retain their national genetics, geography, customs, culture andlife-style.XRCC1genotypes were detected by PCR-RFLP in88patients with EC(Hui38and Han50) and100controls with no cancer in the same area(Hui38andHan50).Results1.The frequencies of the XRCC1codon194C and T allele among ECpatients and healthy controls in Han population were68%32%and67%33%respectively; No significant difference in the XRCC1allele distribution wasshown between EC patients and controls (P>0.05). The distribution of the CC, CTand TT genotypes between EC patients (46%,44%and10%, respectively) andcontrols (60%,34%and6%, respectively) also had no significant difference(P>0.05). The frequencies of the XRCC1codon194C and T allele among ECpatients and healthy controls in Hui population were77.6%22.4%and72%28% respectively; No significant difference in the XRCC1allele distribution wasshown between EC patients and controls (P>0.05). The distribution of the CC, CTand TT genotypes between EC patients (60.5%,34.2%and5.2%, respectively) andcontrols (56%,32%and12%, respectively) also had no significant difference(P>0.05).2. The frequencies of the XRCC1codon280G and A allele among EC patients andhealthy controls in Han population were76%24%and81%19%respectively; Nosignificant difference in the XRCC1allele distribution was shown between ECpatients and controls (P>0.05). The distribution of the GG, GA and AA genotypesbetween EC patients (58%,38%and6%, respectively) and controls (64%,34%and2%, respectively) also had no significant difference (P>0.05). The frequenciesof the XRCC1codon280G and A allele among EC patients and healthy controls inHui population were89.5%10.5%and77%23%respectively; No significantdifference in the XRCC1allele distribution was shown between EC patients andcontrols (P>0.05). The distribution of the GG, GA and AA genotypes between ECpatients (81.6%,15.8%and2.6%, respectively) and controls (54%,46%and0,respectively) had obvious significant difference (P>0.05).3. The frequencies of the XRCC1codon399G and A allele among EC patients andhealthy controls in Han population were69%31%and70%30%respectively; Nosignificant difference in the XRCC1allele distribution was shown between ECpatients and controls (P>0.05). The distribution of the GG, GA and AA genotypesbetween EC patients(48%,42%and10%, respectively) and controls(52%,36%and12%, respectively) also had no significant difference (P>0.05). Thefrequencies of the XRCC1codon399G and A allele among EC patients andhealthy controls in Hui population were76.3%23.7%and80%20%respectively;No significant difference in the XRCC1allele distribution was shown between EC patients and controls (P>0.05). The distribution of the GG, GA and AA genotypesbetween EC patients(60.5%,31.6%and7.9%, respectively) and controls(68%,24%and8%, respectively) also had no significant difference (P>0.05).Conclusions1. The XRCC1codon199280and399SNPs might not be relatedto t he risk of EC development in Han population in Ning Xia area2. The XRCC1codon199and399SNPs might not be related to the risk of ECdevelopment in Hui population in Ning Xia area3. The XRCC1codon280SNPs might be related to the risk of EC development inHui population in Ning Xia area...