| Objective To explore the effects of ghrelin on hippocampal neurons after seizuresinduced by kainic acid (KA), and to investigate the correlation about the changed expressionof ghrelin on hippocampal gamma-aminobutyric acid (GABA) in rats.Method To establish a model of kainic acid induced by epilepsy in rats, and to intervenethe effects of injection with exogenous ghrelin. Ethological and morphological observationswere observed, HE staining and Nissl staining were used to detect the Antiepileptic effects.Immunofluorescence were used to detect the expressions of GABAResults After Kainic acid injection, the rats began to seizure after a latent period.Ghrelin treatment group latency time (12.3±3.5min) was lengthened significantly ascompared with Epilepsy group latency time (8.7±1.2min)(P<0.05), moreover, tonic-clonicduration (10.8±1.4min) was significantly lower than Epilepsy group (15.2±2.7min)(P <0.05).There was an obvious change in hippocampal neurons morphocytology of epileptic rats byHE staining and Nissl staining which also showed severe neuronal loss, and statisticalanalysis showed that the nerve cell apoptosis and loss within Ghrelin treatment group wasdecreased compared with Epilepsy group at different time points (P <0.05).Immunofluorescence detection statistics results showed that the number of GABA neuronswas continued to decrease with time as compared with the control group after injection ofkainic acid in rat hippocampus within24hours. And its decrease trend is more gentle afterinjecting Ghrelin while there were remarkable differences between the two groups (P <0.05).Conclusion Our results implied that ghrelin had neuron protective effects which wereinterrelated through influence on expression of GABA in central nervous system. |
PDF Full Text Request