Effect Of Succinic Acid On The Physiological Characteristics Of Purkinje Cells In Neonatal Rats With Seizures

A neonatal epileptic seizure is common neurological symptoms and the risk ofepileptic seizure is highest in the infants. Infants with a history of seizure often havelong-term neurologic sequelae compared with other age groups of children.Phenobarbital was the first drug that has been used to treat epileptic seizure of infants.Phenobarbital can successfully control of seizures, it may be have recent or long-termdamage to the nervous system for a newborn at the same time.Modern pharmacology study have found that succinic acid is the main activeingredient in amber which is a traditional anticonvulsant. But whether succinic acid havelong-term function on neurons in immature rats after seizure, which have yet to see thereports. Therefore this study to research the effect of a single high-dose of succinic acid onthe physiological characteristics of cerebellar Purkinje cell(PC)in immature rats afterseizure. This study want to provide experimental basis for the succinic acid which is usedsafety in clinical treatment of neonatal epileptic seizure. The recent effect of a single high-dose of succinic acid on the excitabilityof PC in neonatal rats with seizuresObjective: To study the recent effect of a single high-dose of succinic acid on theexcitability of PC in neonatal rats with seizures.Methods:7days after the birth of Sprague-Dawley rats were randomly divided intonormal control group, seizures control group, phenobarbital control group and succinicacid group. The threshold of action potential of PC and its fire rate were recorded bywhole cell patch clamp on cerebellar slices. The acute effect of a single large dose ofsuccinic acid on the excitability of PC of neonatal rats with seizures was analyzed by thistest.Results: Succinic acid increased the threshold of action potential of PC anddecreased its fire rate, compared to seizures control group was statisticallysignificant(P<0.05); compared to normal control group had no statistical difference(P>0.05); compared to phenobarbital control group had no statistical differencetoo(P>0.05).The long-term effect of a single high-dose of succinic acid on theexcitability of PC in neonatal rats with seizuresObjective: To study the long-term effect of a single high-dose of succinic acid onthe excitability of PC in neonatal rats with seizures.Methods:7days after the birth of Sprague-Dawley rats were randomly divided intonormal control group, seizures control group, phenobarbital control group and succinicacid group. Neonatal epileptic seizure were induced by pentetrazole and a single high-doseof PB and succinic acid were applied for seizure rats. All rats were feeding to30days ofold. The threshold of action potential of PC and its fire rate were recorded by whole cellpatch clamp on cerebellar slices of30days of the rats. The long-term effect of a singlelarge dose of succinic acid on the excitability of PC of neonatal rats with seizures wasanalyzed by this test. Results: Succinic acid increased the threshold of action potential of PC anddecreased its fire rate, compared to normal control group had no statistical difference(P>0.05); compared to seizures control group was statistically significant (P<0.05);compared to phenobarbital control group had statistical difference too(P<0.05).The effect of a single high-dose of succinic acid on the synaptic plasticityof PC in neonatal rats with seizuresObjective: To study effect of a single high-dose of succinic acid on the synapticplasticity of PC all rats which were included7days of neonatal rats and30days of therats.Methods: PC excitatory postsynaptic current(EPSC) long term depression(LTD)were recorded by whole cell patch clamp on cerebellar slices of7days of neonatal rats and30days of the rats. The effect of a single large dose of succinic acid on the PC LTD in thecerebellar of all groups was analyzed by this experiment.Results: A single high-dose of succinic acid had no adverse effect on synapticfunctions of PC in the cerebellar of7days of neonatal rats with seizures, compared tonormal control group had no statistical difference(P>0.05); compared to seizures controlgroup had no statistical difference too(P>0.05); compared to phenobarbital control groupwas statistically significant(P<0.05). A single high-dose of succinic acid had not changedthe synaptic functions of PC in the cerebellar of30days of the rats, compared to normalcontrol group had no statistical difference(P>0.05); compared to seizures control grouphad statistical difference(P<0.05); compared to phenobarbital control group wasstatistically significant too(P<0.05).Conclusions:1. Succinic acid decreased the excitability of PC in neonatal rats with seizures.2. Succinic acid had no long-term adverse effect on the excitability of PC in neonatalrats with seizures.3. Succinic acid had no bad influence on the synaptic plasticity of PC of in neonatalrats with seizures and Puberty pups.