Effects Of SiRNA Targeting BubR1on The Biological Behaviors About HBV(+) Hepatocellular Carcinoma Cells

Objective:Effects of siRNA Targeting BubR1on the Biological Behaviors about HBV(+)Hepatocellular Carcinoma Cells.Methods:1. Three different interference siRNA sequences were devised and offered byInvitrogen Corporation According to BubR1gene sequence library.2.Detecting the highest BubR1mRNA expression in five different hepatomacells (HBV-negative and HBV-positive) by RT-PCR,which serves as a follow-up testsubjectsts3. Experimental groups were divided into five,detecting the expression ofBubR1in HCC cells after interferring BubR1by western blot.4. Detecting cell proliferation, colony formation, apoptosis, and the cell cycleafter siRNA transfection by MTT, colony formation and flow cytometry.Results:1.The BubR1mRNA was up-regulated in HBV-positive lhepatoma cell linescomparing to the HBV-negative carcinoma cell lines,especially in HepG2.2.15.2.The BubR1protein in normal and negative group was significantly higherthan in the intervention group (C, D, E)(p <0.05)，which was the lowest in groupE.The difference was statistically significant.3.The cell proliferation was inhibited in intervention group comparing to thenormal group and negative group after interferring BubR1,especially in groupE,which was statistically significant (p <0.05).The apoptosis rate was increased inintervention group comparing to the control group (normal group and negativegroup),especially in group E,which was statistically significant (p <0.05). Cells in S,G2phase were increased in intervention group comparing to the control group(normal group and negative group),especially in group E,which was statisticallysignificant (p <0.05). Conclusion:1.The BubR1protein was down-regulated in HepG2.2.15after interferringBubR1in vitro.2.Cell growth, proliferation and cycle of HepG2.2.15were affected afterinterferring BubR1in vitro.Cell proliferation was reduced, apoptosis ratio was raisedand cell cycle was arrested at G2/M phase.