The Effect Of Livin Modified BM-MSCs Transplantation On Cardiac Function Following Acute Myocardial Infarction In A Rat Model

Objective:To study The effect of Livin gene modified BM-MSCs transplantation on cardiacfunction following acute myocardial infarction in a rat model and the expression oflivin,caspase3,caspase7,and caspase9in the livin gene modified BM-MSCs.Methods:(1). The MSCs were gained by the whole bone marrow culture method and wereidentificated by the expression of its surface markers CD29,CD34,CD45,and CD90by flow cytometry, which also was used to detect the apoptosis of the MSCs afterinfected with adenovirus vector carrying enhanced green fluorescent protein (EGFP)gene and Livin recombinant vector (rAd-livin).(2). The expression of Livin, caspase3, caspase7and caspase9was detected bywestern bolt.(3). after permanent left anterior descending artery occlusion, rats wererandomized (n=10per group) to receive intra-myocardial injections of100microLof DMEM without cells (group vehicle) or containing1million MSCs(group MSCs)or MSCs(EGFP)(group rAd-control/MSCs) or MSCs(Livin)(group rAd-livin/MSCs) cells, and the cells used for translation were stained by DAPI.(4). left ventricular systolic pressure（LVSP），Left ventricular-diastolicpressure(LVDP)，the maximum increased rate of left ventricular pressure(-dp/dtmax)and the maximum decline rate of left ventricular pressure (+dp/dtmax), which wererecorded by physiological recorder, were used for evaluating the cardiac function;and the cells stained by DAPI in the heart were measured by the Fluorescencemicroscope.Results:(1).The apoptosis of rAd-livin/MSCs was significantly declined when comparedto MSCs and rAd-control/MSC（sP <0.05）, meanwhile, the expression of caspase-3,caspase-7and caspase-9was significantly downregulated when compared to twoother groups（P <0.05）； (2).The cardiac function of rAd-livin/MSCs group was significantly improvedwhen compared to the DMEM group,and the other two groups got the similarlyresults,but the function of the rAd-livin/MSCs group was better improved,meanwhile, the number of surviving cells from rAd-livin/MSCs group wassignificantly improved when compared to two other groups.Conclusion:(1).The apoptosis of MSCs can be brought down after the effection ofrAd-livin,and the expression of caspase-3, caspase-7and caspase-9was significantlydownregulated meanwhile.(2).Transplantation with Livin modified BM-MSCs by lentiviral vector leads tobetter prognosis for MI by enhancing cellular survival.