The Process Study Of Synthesis Of Deuterated Amino Acid

Objective The simple, efficient and effective method for the synthesis of deuteratedamino acids were explored, which could cost reduction and produce amino acid, neonatalamino acid metabolism for testing platform were established.Methods Deuterated amino acids were synthesized by D2O exchange.(1) The effect ofsynthesis L-[α,2,4-2H3]-histidine was explored by single factor experiment with reactiontemperature, reaction time and the number of exchanges.(2) L-[α,2,4-2H3]-histidineresponse surface method was optimized by the experimental conditions for L-[α,2,4-2H3]-histidine single factor.(3) Thingking of L-[α,2,4-2H3]-histidine response surface, L-[α-2H]-glutamic and L-[α-2H]-methionine response surface were optimized basing on amino acids,alkali, neutral.(4) According to the response surface optimization conditions, L-prolinesequence, L-glycine, L-isoleucine and L-valine were synthesised.1H-NMR test method wasapplied to calculate the rate of deuterated amino acids. Configuration deuterated changeswere identified by circular dichroism and qualitative analysis by MS.Results (1) The condition of response surface optimization of L-[α,2,4-2H3]-histidinewas optimized including that the reaction time was6.47hours, the reaction temperature was185℃, exchange times was3times.(2) The condition of response surface of L-[α-2H]-methionine was optimized including that the reaction time was6.23hours, the reactiontemperature was182℃, exchange times was3times.(3) The condition of response surfaceof L-[α-2H]-glutamic was optimized including that the reaction time was7.15hours, thereaction temperature was189℃, exchange times was3times.(4) The deuterated rate of L-proline, L-glycine, L-isoleucine and L-valine were synthesised which texted by1H-NMR areall above98.5%, configuration changes are identified by circular dichroism. The compoundsof molecular weight were obtained by using MS.Conclusion (1) The experiment for synthetic amino acid deuterated was used D2Oexchange, which was a simple, efficient and economical method, and can provide thefoundational conditions for future mass production.