Study On The Interaction Between Small Molecules And Bovine Serum Albumin And The Effect Of Nanomaterials On This Interaction

Serum albumin (SA) is a very important body protein in the human body, which has many significantfunctions, including combining with drug, absorbing the small molecule and harmful molecule contain infood and so on. Nanomaterials, for their unique size, have many excellent properties when compared withother materials. They have now been widely used in many fields, including medical researches, industrialproduction, food safty, etc. In the medical area, using nanomaterials as drug carriers is a very popularresearch topic recently. In this paper, we studied the interaction of several common small molecules,including the anticancer drug-resveratrol, the harmful molecule-nicotine and iron supplement-hemin withbovine serum albumin (BSA) from the molecular level. We got the binding information of several substanceswith SA by fluorescence spectroscopy, and also introduced the nanoparticles to investigate the possiablechange caused by the nanoparticles. The presented thesis can be described in three parts as following:1. Experiment was mainly focused on the reaction of nicotine with BSA, and then added Agnanoparticles in the reaction. By comparing the results concluded before we found that Ag nanoparticlescould inhibit the binding of nicotine and BSA. In order to investigate the mechanism of the inhibition, wealso used several spectroscopic methods to study the combination of BSA and Ag NPs, the results show thatthe combination of Ag NPs with BSA will lead to the changes in secondary structure of BSA, therebychanged the binding of nicotine and BSA.2. We use fluorescence spectroscopy, UV absorption spectroscopy and differential pulse voltammetry tostudy the interaction of BSA and iron supplement hemin, then compared with the result drawn when tannicacid was added in the same experimental system. According to the ontained results, the binding constant andnumber of binding sites of BSA and hemin would decrease after the addition of tannic acid, and the changeof secondary structure of BSA was also weaker. On the other hand, we research the influence of tannic acidon the binding force between hemin and BSA, and draw the conclusion that tannin does not change the typeof binding force between hemin and BSA.3. We synthesized and characterized PEG-Fe3O4nanoparticles according to the literature, research theinteraction of anticancer drug resveratrol and PEG-Fe3O4by fluorescence and UV spectroscopy. It showedthat the nanoparticles will react with resveratrol and obtain the drug loading particle. Then we studied theinteraction between nano materials and BSA by spectroscopy and chemometrics, and study the effectsPEG-Fe3O4may cause, proposed the possibitly of PEG-Fe3O4used as a drug carrier.