Association Study Of The FGFR2Gene Polymorphism And Breast Cancer Susceptibility In The Qinghai Tibetan Population

Objective The Morbidity of breast cancer increased rapidly year by year, and nowbreast cancer has become one of the most common cancer in Chinese women.Tibetans also found an upward trend in the incidence of breast cancer, which has beenranked at least four of the nation malignancy. Different from hereditary breast cancer,often sporadic breast cancer is the result of interact between genetic susceptibilitybackground and environmental factors. A number of studies show that, FGFR2genepolymorphism multiple sites associated with breast cancer, rs2981582which islocated in FGFR2gene intron2relevance to breast cancer strongest. Qinghai is amulti-ethnic gathering; Tibetan is one of the relative isolation of the main ethnicgroups.At present, relevant research for FGFR2gene polymorphisms and breastcancer are mainly concentrated in the Han population, lack of minority genetic data.This study aims to understand the correlation between FGFR2rs2981582polymorphism and breast cancer susceptibility in Qinghai Tibetan population, toaccumulate the isolated ethnic population genetics data.This study aims to analyze theFGFR2rs2981582locus genotype and allele frequency distribution in the QinghaiTibetan breast cancer patients and the normal population by PCR and DHPLCtechnology; to investigate the correlation between FGFR2rs2981582polymorphismand breast cancer susceptibility in Qinghai Tibetan population.Methods This is a case control study, We randomly selected the peripheral blood ofthirty-five patients with breast cancer and corresponding thirty-five healthy person,and extracted their DNA standby, Calculate the purity and concentration,1.5%agarose gel electrophoresis its integrity. DHPLC were used to detect the FGFR2geneloci (rs2981582) after PCR amplification of each sample, sequencing confirmed abnormal peak.Then we analyzed its genotype and allele frequency distributionbetween patients with breast cancer and healthy person, discussing its relevance tobreast cancer susceptibility.χ2test were used to clear differences between genotypeand allele frequencies in the study group and the control group, P <0.05wasconsidered statistically significant; with an odds ratio (OR) and95%confidenceintervals (95%CI) represents the relative risk.Results CC, CT, TT genotype frequency distribution of study group was25.71%,28.58%,45.71%, T allele frequency distribution was60.00%; CC, CT, TT genotypefrequency distribution of control group was37.14%,51.43%,11.43%, the T allelefrequency distribution was26.00%.After Hardy-Weinberg equilibrium genetic testing,genotype and allele frequency distribution of FGFR2rs2981582were in line with theHardy-Weinberg equilibrium both in genotype study group and the control group.Genotype and allele frequency distribution in the study group and the control groupwere statistically significant difference (P=0.006and0.007, respectively).Conclusion There was statistically differences in the genotype frequency and allelefrequency between breast cancer group and the control group (P<0.05),and carring Tallele is an increased risk of breast cancer （OR=2.538，95%CI=1.285-5.015）.