| Objective: To observe the effects of sodium hydrosulphide (NaHS) on chronic neuropathicpain and channel protein pNR2B, pCaMK II and pCREB expression in chronic constrictioninjury the sciatic nerve SD rats (CCI) and investigate NaHS the analgesic mechanismmechanism in the CCI spinal nociceptive transmission.Methods:108male SD rats,180-200g were randomly divided into6groups,18rats in eachgroup:(1)control group: there was not any operation treatment;(2)sham operation group(sham group): only do blunt separation, exposed the sciatic nerve, no nerve ligation;(3)CCIgroup;(4)CCI+NaHS small group (NaHS,0.375mmol/kg/day; NaHSSgroup);(5)CCI+NaHS middle group (NaHS,0.75mmol/kg/day; NaHSMgroup);(6)CCI+NaHS large group(NaHS,1.5mmol/kg/day; NaHSLgroup). The rats were measured with thermal pawwithdrawal latency (PWTL) and the mechanical withdrawal threshold (MWT).Immunohistochemistry was used to detect the expression of pCREB in the spinal dorsal hornof rats. The levels of pNR2B and pCaMKII was detected by using western blotting.Results:1, The changes of behavior: PWTL and MWT were no statistically significantdifferences of bilateral hind limbs in all rats before operation(P>0.05); Compared with controlgroup, PWTL and MWT in sham group was no significant difference (P>0.05); Comparedwith sham group, postoperative1d,3d,7d and14d, the PWTL and MWT were decreased in atime dependent manner, postoperative14d, PWTL and MWT were markedly decreased(P<0.05); NaHS inhibited reducing of PWTL and MWT in CCI rats in a dose-dependentmanner. PWTL and MWT were markedly higher in NaHSMgroup than that in CCI group.2, Immunohistochemical staining showed: The expression of pCREB in spinal dorsal hornwas no significant difference between control and sham group (p>0.05); Compared with shamgroup, the expression of pCREB of spinal dorsal horn in CCI group rats was increased in atime dependent manner, In CCI14d, pCREB was increased significantly campared to that insham group (p<0.05). Compared with the CCI group, NaHS inhibited the level of pCREB inconcentration dependent manner, level of pCREB was greatly decreased in NaHSMgroup(p<0.05). 3, Western blot showed: The expressions of pNR2B and pCaMKII in spinal dorsal horn wereno significant difference between control and sham group (p>0.05); Compared with shamgroup, the expression of pNR2B and pCaMKII of spinal dorsal horn in CCI group rats wereincreased in a time dependent manner, In CCI14d, pNR2B and pCaMKII were increasedsignificantly campared that in sham group (p<0.05); Compared with the CCI group, NaHSinhibited the level of pNR2B and pCaMKII in concentration dependent manner, level ofpNR2B and pCaMKII were greatly decreased in NaHSMgroup (p<0.05).Conclusion:1, NaHS could improve the threshold of PWTL and MWT in CCI SD rats;2, NaHS could inhibit the expression of pCREB, pNR2B and pCaMKII in spinal dorsal hornof CCI SD rats;3, The effect alleviating NNP in CCI rats by NaHS may be related to inhibiting the activity ofpNR2B and pCaMK II/pCREB in CCI spinal nociceptive transmission. |
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