| ObjectiveThe purpose of this study to reveal the relationship between pregnant womenwho inhaled oxygen concentration in the perinatal period and between retinopathyof premature,By giving late pregnant rats inhalation of high concentration of oxygenand observing development of vascular in neonatal rat retinal, establish high oxygeninduced animal models of retinopathy of premature.MethodsChoose40gestational age C57BL/6mice about16days pregnant,these ratswere randomly divided into four groups, each group of10, Control group:10pregnant rats raised in the air, Experimental group (Group1):10gestational age of16pregnant rats were housed in75%oxygen tank, after pregnant rats production,stop offerring oxygen, put the female rats together with the newborn rats exposed toair. Experimental group (Group2):10pregnant rats breeding in air, until the7th dayof newborn rats born, put the female rats together with the newborn rats in75%oxygen tank. Experiment3(Groups3):10pregnant rats on the16th gestational agebreding in75%tank, until newborn rat age of7days, put the female rats exposed toair together with newborn rats.To understand the changes of retinal blood vessels byimmunofluorescence, count the membrane of vascular endothelial cell nucleus whichbreakthrough internal retina,by HE staining of eye tissue. observe the retinal’sexpression of vascular endothelial growth factor (VEGF) and transforming growthfactor β1(TGF-β1)Results1.The counting statistics HE staining breakthrough the internal limitingmembrane, The number of endothelial cell break the internal limiting membrane inthe control group is (1.30±1.10), experimental group1, group2, group3,respectively (8.53±5.00),(9.34±6.80),(18.20±11.60). compared with the experimental group P <0.05; contrast experimental group1and experimental group2, P>0.05, not statistically significant. Experiment3group and the experimentalgroup1, group2comparison, P <0.05, statistically significant differences.2.Immunohistochemistry in paraffin sections shows: control group of VEGFand TGF-β1-positive samples were integrated optical density (70.89±61.94),(25.34±11.23); compared with the experimental group, P <0.05, statistically significant.Experiment1group and experimental group2, group3compared, P <0.05, there isstatistically significant. Experiments group2compared with experimental groups3,P>0.05, no significant difference in the two groups.3.Retinal preparation shows: There is significant difference test three groupsand other groups.Conclusions1.Pregnant rats inhaled oxygen concentration may induce new blood vesselsand fibrous proliferation of newbron ratts, conform to the pathological mechanism ofretinopathy of premature.2.This methods are effective, rational and scientific. which was constructed inretinopathy of preterm and hyperoxia-induced animal model of retinopathy ofpremature.3.The pregnant woman inhaling high concentrations of oxygen in the perinatalperiod may induce or cause retinopathy of premature,there may be a certainrelationship. |
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