Expression And Significance Of Regulatory T Cells And Th17Cells In The Spleen In Idiopathic Thrombocytopenic Purpura(ITP)

Objective: To investigate the expression of Regulatory T cells and Th17cells in the spleen in idiopathic thrombocytopenic purpura (ITP) and theirsignificance.Methods:43ITP patients performed splenectomy in Fujian MedicalUniversity Union Hospital during2008-2012were enrolled by thediagnostic criteria for ITP.The control group consisted of10patientswho performed splenectomy because of splenic impairment. The case groupwas further divided into effective group and ineffective group accordingto the effect after operation. The quantity of regulatory T cells and Th17cells in the spleen from patients and controls were examined withImmunohistochemistry(IHC).All data were analyzed by SPSS17.0.Results: The quantity of regulatory T cells in the spleen of ITP patientswas significantly lower than the normal controls(P<0.01),but there wasno statistical differences between effective group and ineffectivegroup(P>0.05).The quantity of Th17cells in the spleen of ITP patientswas markedly higher than that of the normal controls(P<0.01),while therewas no statistical differences between effective group and ineffectivegroup(P>0.05).Treg/Th17of ITP patients was significantly lower than thenormal controls(P<0.01).Conclusions: The reduction of regulatory T cells and the increase of Th17cells do exist in the spleen of ITP patients and lead to a loss of balanceof Treg/Th17, that may play an important role in the pathogenesis anddevelopment of ITP.The degrees of regulatory T cells and Th17cells inthe spleen between effective group and ineffective group are no statistical differences.