The Significance Of Th17and T Regulatory Cells In Idiopathic Thrombocytopenic Purpura

Objective:This study examined The percentage of Th17cells and CD4+CD25+regulatory T cells (Treg) accounted for CD4+T cells and the expression of platelet antibodies IgG in peripheral blood of idiopathic thrombocytopenic purpura (ITP) patients, verified and investigated the role and the significance of Th17and T regulatory cells and the imbalance of Th17/Treg cells in ITP; and studied on the significance of the correlation between Th17cells and platelet-associated antibodies in ITP.Methods:Its35cases of ITP patients (both naive) who were in treatment or in hospital treatment of Lanzhou University Second Hospital and Lanzhou University First Hospital from December2009to December2012, and35healthy subjects over the same period were selected as the objects of this study. All specimens were collected fasting peripheral blood and anticoagulated by heparin sodium and EDTA. By flow cytometry (FCM), the percentage of Th17cells and CD4+CD25+regulatory T cells (Treg) accounted for CD4+T cells in vivo of ITP patients and healthy controls were determined; the level of platelet-associated IgG antibodies in plasma of ITP patients and healthy controls were determined by the enzyme-linked immunosorbent assay (ELISA).Result:The percentage of Th17cells in peripheral blood of patients with ITP is higher than that of the healthy control group, and the results are statistically significant difference (P<0.05). The percentage of CD4+CD25+regulatory T cells in the peripheral blood of ITP patients is significantly lower than that of the healthy control group, and the results are statistically significant difference (P<0.05). The level of platelet-associated IgG antibodies in the plasma of patients with ITP is significantly higher than that in the control group, and the results are statistically significant difference (P<0.05). The expression between Th17cells and platelet-associated antibody in peripheral blood of ITP patients has no obvious correlation, and the correlation coefficient is-0.21. The ratio of Th17/Treg cells in the bodies of the ITP patients is higher than that of the healthy control group, and the results is statistically significant difference (P<0.05).Conclusion:The percentage of Th17cells accounted for CD4+T cells and the expression of platelet antibodies IgG in vivo of ITP patients are increased and there are no significant correlation between them. The percentage of CD4+CD25+regulatory T cells (Treg) accounted for CD4-T cells is decreased. Thl7/Treg cells increased significantly. Th17and T regulatory cells and the expression of platelet-associated antibodies IgG in vivo of patients with ITP may play an important role in the pathogenesis of ITP; and the imbalance of Thl7/Treg cells may be important in the pathogenesis of ITP.