The Function Of Inhibiting VEGFR2by Gambogic Acid In Mechanism Of Anti-colorectal Cancer

Objective:This paper intends to test gambogic acid influence on the vascular endothelialgrowth factor receptor2(VEGFR2) of the tumor microvascular-related gene, to probeinto the relationship between VEGFR2and the microvascular density (MVD), toexplore gambogic acid resistant mechanism against colorectal cancer, and toprovide theoretical basis for the clinical use of gambogic acid to treat colorectalcancerMethods:This research intends:1) to use animal experimental method to examine theeffects of different doses of gambogic acid on the tumor-loaded nude mice toinvestigate the inhibitory effect of gambogic acid on the tumor and calculate theinhibition rate of tumor;2)to use HE staining method to observe the microvascular changes and analyze the impact of gambogic acid on the mentioned index aswell as the relationship between VEGFR2and microvessel density3) to utilizeimmunohistochemical method to detect microvessel density and VEGFR2expressionin the tumor tissue;1) to adopt the fluorescence RT-PCR to detect differentconcentrations of gambogic acid on VEGFR2mRNA transcription level in tumortissue; Results:The animal experimental results show that the increase of gambogic acid dosehas significant inhibitory effects on tumor growth reduce blood nutrition supply leadto coagulation necrosis of tumor and reduce the expression of tumor tissue VEGFR2as well as tumor’s microvessel density, thus inhibiting tumor angiogenesis to exertanti-tumor effects.The different concentrations of gambogic acid with their increaseexerts more obvious influence on the surface of VEGFR2mRNA transcriptioninhibition of tumor tissueConclusion:The acid is an effective component extracted from Chinese traditionalmedicine,gambogic acid, a broad-spectrum anti-tumor drug. It has a significant effecton many kinds of cancer, especially excellent efficacy in colorectal cancer."Tumorangiogenesis hypothesis" holds that tumor growth depends on angiogenesis; theinhibition of vascular endothelial growth factor receptor2(VEGFR2) can cause theintracellular Wnt/-Catenin, FAK, c-Src, AKT and other changes in signalingpathways, then ultimately inhibit angiogenesis, and further prevent tumor. The studydemonstrates that gambogic acid has has significant inhibitory effects on tumorgrowth reduce blood nutrition supply lead to coagulation necrosis of tumor vivostudies indicates that gambogic acid can reduce the expression of tumor tissueVEGFR2protein, thus reducing tumor microvascular density and inhibiting tumorangiogenesis to exert anti-tumor effects. inhibit VEGFR2mRNA transcription thenreducing biological effect of VEGFR2lead to inhibition of t microvessel growth intumor tissue reduction of microvessel density to exert anti-tumor effects.