| Background: Colorectal carcinoma is one of the most common cancers. Its mortality ranks the third among all cancers, just inferior to lung cancer and gastric cancer in the world. It harms people's health fatally. So it is important to look for markers which have close relationship with the biological behavior of colorectal cancer, and to recognize colorectal carcinoma which has the high potential to invade and metastasize. The carcinogenesis development and metastasis of colorectal carcinoma is a multistep process and is affected by many factors. Recently, people pay more attention to the angiogenesis of tumor. The angiogenesis of tumor provide the tumor tissue with nourishment and air to exchange. It's also the most direct way that tumor metastasizes. c-Met, a protein product of met proto-oncogene, is a tyrosine kinase receptor for the hepatocyte growth factor (HGF). HGF/SF-met signaling has multifunctional effects on mammalian cells, including stimulation or inhibition of cellular proliferation, promotion of cell movement, invasion into extracellular matrix, and induction of glandular/tubular morphogenesis by epithelial cells. Activation of c-Met promotes tumor cell proliferation, dissociation, invasiveness and angiogenesis. Aberrant expression of c-Met may play a role in tumor progression.VEGF is a high specific fission-promoting factor of vascular endothelial cells. It can stimulate the proliferation of endothelial cell, promote angiogenesis and growth of tumor,make the condition for tumor's invasion and metastasis by enhancing the penetration of microvessel and direct acting on the specific receptor of vascular endothelial cell. MVD is an important parameter which reflects the biological behavior of malignant tumor. It has a close relationship with the metastasis of tumor. Nowadays the most specific CD34 is usually be used to mark vascular endothelial cell. Up to now the study of c-Met and VEGF expression and MVD in colorectal carcinoma has not been much reported.Objective: To investigate the expression of c-Met, VEGF in colorectal carcinoma and their roles in carcinogenesis, invasion and metastasis of colorectal carcinoma as well as their relationship with angiogenesis and the significance of clinicopathology, provide new reference for forecast the carcinogenesis, invasion and metastasis of colorectal carcinoma, and judge prognosis.Materials and methods: Fifty one cases of surgically resected colorectal adenocarcinoma samples, sixteen cases of adenoma samples and sixteen cases of colorectal normal tissue samples were all confirmed by pathologically. Investigate the expression level of c-Met, VEGF, CD34 by using immunohistochemical SP method, counting the microverssel density. MVD was marked by x + s. All of the statistical analysis was performed with the statistical package SPSS version 10.0. Association between parameterswas assessed by t-test, analysis of variance, x2 -test or Fisher's exact test, Spearman stagerelate analysis. P value less than 0.05 indicated statistical significance.Results: 1. The positive rate of c-Met in colorectal carcinoma is 72.55%(37/51), which is significantly higher than that in adenoma(7/16, 43.75%) and colorectal normal tissue(12.5%, 2/16), (P<0.05).2. In colorectal cancer tissue, c-Met positive expression does not correlate with the histological types and tumor site significantly(P>0.05), but has a significant correlation with Dukes stage, depth of invasion, lymph node metastasis and distance metastasis(P<0.05).3. The positive rate of VEGF in colorectal carcinoma is 52.94%(27/51), which is significantly higher than that in adenoma(12.5%, 2/16) and normal tissue(0%, 0/16), (P<0.05).4.In colorectal cancer tissue, VEGF positive expression does not correlate with the histological types and tumor site significantly(.P>0.05), but has a significant correlation with Dukes stage, depth of invasion, node metastasis and distance metastasis(P<0.05).5. The MVD in colorectal carcinoma tissue is (28.67+11.94), which is significantly higher than that in adenoma(...
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