The Preliminary Study Of The Radiosensitivity Of Lung Cancer Cells In Vitro Affected By Human Lung Cancer-associated Fibroblasts

ObjectiveTo investigate the radiosensitivity of lung cancer cells affected by human lungcancer-associated fibroblasts (CAF) in cell to cell contact co-culture in vitro, to investigatethe possiblie influencing factors of lung cancer cells radiosensitivity.MethodsHuman lung CAF was obtained from fresh human lung adenocarcinoma tissuespecimens used by primary culture with tissue explants method, and was identified byimmunofluorescence staining. The radiosensitive differences of A549and H1299lung cancercells were investigated by cell survival curves in cell to cell contact co-culture compared tolung cancer cell culture alone, respectively.ResultsThe human lung CAF was obtained successfully, and could stably grow and passage.The CAF had specific immuno-staining with specific α-SMA, imentin, and FAP, and nocytokeratin-18expression. The plating efficiency (PE,%) of A549cells at0Gy irradiationwere (20.0±3.9)%and (32.3±5.5)%in A549cells culture alone and in cell to cell contactco-culture, respectively (t=3.16, P<0.05). The PE of H1299cells at0Gy irradiation were(20.6±3.1)%and (35.2±2.3)%in H1299cells culture alone and in cell to cell contactco-culture, respectively (t=6.55, P<0.01). The cell survival curve showed that SF2of A549cells were0.727±0.061and0.782±0.089in A549cells culture alone and in cell to cell contact co-culture, respectively (t=0.88, P>0.05), SF2of H1299cells were0.692±0.065and0.782±0.037in H1299cells culture alone and in cell to cell contact co-culture, respectively(t=2.08, P>0.05). The protection enhancement ratio of human lung CAF for A549and H1299cells were1.29and1.25, respectively.ConclusionsHuman lung CAF had radioprotective effect on lung cancer cells significantly. Itseffect may attribute to CAF promoting lung cancer cell proliferation.