Amino Acids And Genetic Basis Of Body’s Total Antioxidant Activity And Effects Of Oxidation On Antibody Activity And Other Laboratory Indicators

ObjectiveIn this study, we investigated the antioxidant capacity of amino acids and protein tounderstand the influencing factors of organic antioxidant activity and also exploredeffects of oxidant on the activity of protein antibody. And meanwhile, antioxidantcapacity, routine biochemical indices and three polymorphic loci (TaqIB、I405V、D422G) within gene of cholesterol ester transfer protein (CETP) gene of randomlyselected patients with diabetes and with cardiovascular problems were measured toexplore the relations among the oxidation-antioxidant system, gene polymorphism,antioxidant capacity and the routine laboratory indicators.Methods1. The redox titration (microtitration of potassium permanganate, microtitration ofiodimetry) and T-AOC kit method were adopted for the determination of antioxidantcapacity of22amino acids in the composition of the protein in vivo.2. The results of antioxidant capacity in our study were combined with the data ofamino acid composition of protein queried from the global protein Knowledge and database of protein primary structure. The statistical software of SPSS13.0was appliedto analyze the above results the antioxidant capacity of protein selected in this studywas acquired.3. Blood samples of140cases of patients with diabetes and cardiovascular problemswere randomly collected. The average age of the patients was59.93±11.31. SpecificPCR (AS-PCR) were detected for CETP gene polymorphism loci (TaqIB, I405V,D422G) of the whole blood samples. Simultaneously, routine biochemical indices(glucose, total protein, globulin, albumin, the ratio of albumin to globulin, alanineaminotransferase, aspartate aminotransferase, urea, creatinine, uric acid, totalcholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoproteincholesterol)of the patients were detected. The redox titration (microtitration ofpotassium permanganate, microtitration of iodimetry) and T-AOC kit method wereadopted for the determination of antioxidant capacity of serum of the patients to explorerelations of the organic oxidation, antioxidant ability and gene polymorphism of CETPand routine biochemical indices.4. Three different oxidizer (potassium permanganate solution, iodine solution, hydrogenperoxide solution) were mixed with serum antibody (ovalbumin, and hepatitis B surfaceantibody) in suitable proportions for a period of time, and then the ovalbumin antibodyand hepatitis B surface antibody were subjected to enzyme-linked immunosorbent assay(ELISA) and results were acquired.Results1. The data measured with three methods was subjected to strike the quadratic curveusing SPSS13.0statistical software. The values of antioxidant ability were obtained bycalculating the area under the curve and then the order of antioxidant ability of22typesof amino acids by the rank order. The results are shown below. Amino acids withrelatively stronger antioxidant capacity are tryptophan, methionine, tyrosine, histidine,lysine and cysteine; amino acids with weak or almost no activity are alanine4-hydroxyphenyl proline, arginine, phenylalanine, proline, threonine, glycine, valine, serine, leucine, glutamine, asparagine, cysteine, isoleucine, glutamic acid and asparticacid.2. The antioxidant capacity of different kinds of human serum proteins (human serumalbumin, bovine serum albumin, ovalbumin, globulin, histone) did not differsignificantly in the level of P <0.05. This means the antioxidant capacity of protein maybe independent of the types of protein.3. The organic antioxidant capacity is related to allele D of CETP peptide gene andcertain serum biochemical indicators.4. Effect of potassium permanganate and iodine on the immune responses of ovalbuminantibody depends on the concentration. They play an inhibitory impact above certainconcentration and below which they will play a minor role in promoting. The influenceof hydrogen peroxide on the immune response of ovalbumin antibody is above a certainconcentration it plays minor role in promoting, but does not show inhibitoryphenomenon. In contrast, for the hepatitis B surface antibody, effects of iodine on theimmune response is similar to ovalbumin antibody. Effects of potassium permanganateon the immune response of hepatitis B surface antibody is in a higher concentration, itplays inhibited impact. Hydrogen peroxide always inhibits the immune response ofhepatitis B surface antibody within a certain range of concentrations.Conclusions1. The antioxidant ability of serum protein may not be irrelevant to the types of protein.2. The organic antioxidant capacity is relate to allele D of CETP peptide gene to someextent. But there might be little relation between organic antioxidant capacity andexpression product of allele D.3. Under certain conditions, oxidative stress may suppress the body’s immune responseand hence bring about hazards.