Preparation And Biological Properties Research Of Electrospinning Silk Fibroin/polycaprolactone Blend Film

[Objective] In this study, the blended fiber film was to be generated with polycaprolactone (PCL) and silk fibroin (SF) by electrospinning in different ratio, to combine the good mechanical strength and favorable biological properties together. The primary biological property would be tested.[Methods] First,the silk fibroin were extracted from raw silk. Then the SF/PCL blend fiber film in different ratio (SF/PCL=3:7,5:5,7:3) were produced by electrospinning. The human embryonic lung fibroblast cell suspension adjusted to1.0×105/ml, were inoculated on blended fiber membrane. After4h culture, the specimens were investigated by scanning electron microscopy (SEM), laser scanning confocal microscope (LSCM) and cell adhesion rate were calculated to evaluate the biological property of the blended fiber membrane.[Results](1) The parameters of electrospinning were adjusted to:12cm distance,20kv voltage,2.0ml/h pump rate,10%solution concentration. It was found when the ratio of SF/PCL=7:3, the best product could be obtained.(2) The MRC-5could adhere on the blended fiber film (SF/PCL blend ratio7:3,5:5,3:7) after4h incubation. At the same time, most of cells extended filopodia, pseudopodia under SEM, indicating the good biological properties conducive to MRC-5’s early adhesion and growth on SF/PCL blend film.(3) As can be seen from the detection of cell adhesion rate,when SF/PCL ratio is7:3, the cell adhesion rate is the best on blend film.[Conclusion] In this study, the SF/PCL composite fiber films were generated by electrospinningin different ratio(7:3,5:5,3:7). When the ratio of SF/PCL equals7:3, the best product and best human embryonic lung fibroblast cells adhension rate could be obtained.