| Objective: Chinese herb drugs (CHD) always produce amazing healing efficacy, onlyif the safety and the controllability(SnC) are achieved. In recent years, while effortsthat have been done in Chinese materia medica by both academia and clinic arenumerous, unsolved problems especially based on the SnC of compatibilityapplication may be much more. Having the SnC processing integratedly is the onlyattitude that allowed. In the condition of compatibility use, reduction of safety riskand ensurance of therapeutic effect should be regarded as equally crucial. The purposeis to discover the common rules by compatibility research on certain pair hoping thatit can provide a guideline for similar application.“Eighteen Incompatibilities” is thecore content of the incompatibility of Chinese herb drugs. Veratrum nigrum L.(VN),also called lilu, a poisonous herb, which is on a part of the Eighteen Incompatibilitieslist called “zhushen xin shao pan lilu”, was actually used quite a few together with itsincompatible partners in ancient times. As the intersection of the SnC of Chinesemateria medica, VN, with its incompatible partners, plays a crucial role in theunderstanding of these incompatibility applications. This study deals with VN and itsincompatible partner Panax ginseng C. A. Mey.(PG), aiming at discoveringfundamental features of virulence enhancement and efficacy interference at both theindividual level and the organ level.Methods: In the section of virulence enhancement, an8-week long term rat toxicitytest was taken in order to investigate the possible changes in behavior, weight,food-water intake, blood cell counting, serum test, urine test and histopathologicalstudy with the co-administration of VN and PG(VN-G).In the other section “efficacy interference”, mouse strain model was designed to testfatigue tolerance, activity of GSH and blood viscosity after drug. The purpose was toobserve the influence of VN on the anti-sport-fatigue effect of PG.Results: In the section of virulence enhancement, results show that VN-G contributed to a lower food intake and a higher water intake. It led to a decrease on white ball cellcouting, an increase in both hemoglobin and platelet counting in rats. Significantdifferences between VN-G and VN administration are observed in serum test resultsincluding hepatic function test(alanine aminotransferase, aspartate transaminase,lactate dehydrogenase, alkaline phosphatase), cardiac function test(aspartatetransaminase, creatine kinase, creatine kinase MB, lactate dehydrogenase,α-hydroxybutyrate dehydrogenase) and renal function test(serum urea nitrogen,sodium level, chloridion level), which indicating VN-G’s higher toxicity over VN.Serum glucose level got higher in high dose VN-G group. Histopathologicalobservation showed that VN-G did more extensive damage than VN did to cerebrum,cerebellum, pituitary, myocardium, hepatic lymphocytes, sinus hepaticus, spleen,renal cortex, kidney tubules and bone marrow.In the other section “efficacy interference”, results show that compared with themodel group, ginseng delayed the fatigue timing, enhanced the activity of GSH,improved the hemorheology condition, which VN-G did the opposites. Interestingly,low-dose VN-G did not show much impact on the anti-sport-fatigue effect of PG.Conclusion: By the8-week long term toxicity test, evidences were foud thatcompared to VN, VN-G(with a dose ratio of1︰2.63) can cause more severe andextensive influences on food and water intake, hepatic, cardiac and renal function,more damages on nervous system, heart, hepar, spleen, kidney and bone marrow inrats, which argues the confirmation of virulence enhancement.In the other section, statistics shows that VN can weaken the anti-sport-fatigue effectof ginseng in mice, indicating the confirmation of efficacy interference. Besides, thisweakening seems to be quite minor at the normal clinical dosage, suggesting apotential controllability of PG’s efficacy interfered by VN in clinical practice. |
PDF Full Text Request