The Study On The Protective Effect Of Ginsenoside Rb₁on The Rat Hepatic Ischemia-Reperfusion Injury

Objective:Preliminary study the protective effect of GS-Rb1on rat hepatic ischemia-reperfusion injury and its dose-effect relationship, in order to provide a theoretical basis for the drug research on prevention and treatment of hepatic ischemia-reperfusion injury.Methods:The healthy male SD rats were randomly divided into3groups (n=10), Group A:control group; Group B:low-dose GS-Rb1(20mg/Kg) group; Group C: high-dose GS-Rb1(40mg/Kg) group. Use Pringle’s method to establish the model of hepatic ischemia-reperfusion injury. Group B, C rats were injected into different doses (20,40mg/Kg) GS-Rb1solution before ischemia by the superior mesenteric vein. Group A rats were given the same volume of saline by the the same method. Loosen the clamp blood vessels liver ischemia-reperfusion after20min. After2h reperfusion, collect the blood in inferior vena cava and cut the liver tissues. Serum levels of ALT> AST and ALP、TBIL were measured. Contents of MDA and activity of SOD were measured. The liver tissue samples placed in the light microscope to observe the pathological changes after HE staining.Results:Compared with control group, serum levels of ALT、AST were significantly decreased in Group B (P<0.05), and compared with group C, serum levels of ALT、 AST were also decreased significantly in Group B (P<0.05). Compared with control group, serum levels of ALT、AST were lower but no statistically significant in Group C (P>0.05). In Group B and C, serum levels of ALP、TBIL were lower than control group, but there is no significant difference between each other. In Low-dose GS-Rb1(20mg/Kg) group, the activity of SOD in serum were significantly higher than control group (P<0.05), while the concentration of MDA dramatically decreased(P<0.05). Liver histopathology showes that, control group has swelling of the liver cells with fatty degeneration, local focal necrosis, central venous congestion and sinusoidal dilatation, no obvious inflammatory cell aggregates in the periportal; While GS-Rb1preconditioning group has scattered steatosis and no necrosis, attenuating pathological lesions compared with the control group; Compared with Group B and C, there is no significant difference.Conclusion:1. GS-Rb1(20mg/Kg) has a protective effect on rat hepatic ischemia-reperfusion injury;Increasing the dose (40mg/Kg) can not make this protective effect enhanced, also find no cases of aggravated liver tissue damage.2. The protective mechanisms that GS-Rb1on rat hepatic ischemia-reperfusion injury, may be related with reducing the generation of ROS in the process of ischemia-reperfusion, thereby attenuating tissue damage caused by lipid peroxidation.