Decellularization+Glutaraldehyde+Heparin Were Applied To Reduce The Immunogenicity Of Porcine Aortic Valves

Objective:The advantages of bioprosthetic heart valves are no noise, no hemolysis, no thrombosis, good hemodynamics and need not long-term anticoagulant therapy. However, there are also some disadvantages such as poor durability and easy failure. The immunogenicity of bioprosthetic heart valves is the main reason causing calcification which plays an important role to its failure and comes more and more into focus. In our study, porcine aortic valves were treated with decellularization+glutaraldehyde+heparin binding. Then its immunogenicity was evaluated. The aim of the study is exploring new ways to prevent calcification of bioprosthetic heart valves.Methods:The fresh porcine aortic valves were divided into A, B, C, D groups. A:fresh porcine aortic valves; B:valves decellularized by detergent extraction and enzymatic(DEE); C: valves treated with decellularization+GA; D:valves treated with decellularized+GA+heparin. The extract of each group’s valves is used as antigen to stimulate murine spleen lymphocytes produced cell supernatant containing chemotactic factor. Calculate the mobility of each group’s cell supernatant in Transwell migration. The result was analyzed by SPSS17.0.Results:The mobility of each groups:A:45.2804±3.5841%; B:18.4329±1.3699%; C:11.8626±1.0043%; D:8.7693±0.7162%. Using SPSS17.0to analyse the results by one-way ANOVA, homogeneity of variance test:F=2.643, P=0.075, variance not neat. The P between each gruop:A and B:P=0.002, A andC:P=0.002, A and D:P=0.002, B and C:P=0.004, B and D:P=0.001, C and D:P=0.032. The difference between the groups was statistically significant.Conclusion:Compared with the other three methods, the method of decellularization+GA+heparin binding achieved the best effect on reducing the immunogenicity of porcine aortic valves.