| Objective:To study the basis of obstructive jaundice early application of recombinant human growth hormone (recombinant human growthhormone, rhGH) experiments on the use of obstructive jaundice rat model to simulate the clinical and pathological physiological mechanisms in patients with obstructive jaundice, intestinal mucosal epithelial injury research Claudin (tight junction associated protin, TJP) claudin-3and protein kinase-A (protein kinase A, PKA) expression in rats with obstructive intestinal mucosa, and to explore its mechanism.Methods:63healthy wistar rats, male or female, were randomly divided into five groups:Ⅰ=sham group (sham operated, referred to as the SO group) totaling15; Ⅱ=bile duct ligation (common bile duct ligation, referred CBDL) seven days a total of12group; Ⅲ=CBDL14day group totaled11; Ⅳ=CBDL+rhGH (referred to rhGH group) seven days a total of13group; Ⅴ=rhGH14groups totaling12days. CBDL group and rhGH group after anesthesia with4.0silk free the common bile duct ligation, so that the common bile duct is blocked, made obstructive jaundice model group. rhGH group to begin daily intraperitoneal injection of rhGH from the first day after surgery (Shanghai Fumengjiyin Biosciences Limited offer), SO CBDL group to group and normal saline as a control. Experiment7days and14days, respectively, to detect the changes in liver function and application of immunohistochemistry, semi-quantitative analysis by light microscopy to observe the morphological changes in the distal ileum of the small intestine mucosa and observations tight junction protein claudin-3and PKA at the end back to the farm epithelial expression of cells. Application SPSS17.0statistical software for statistical analysis of the data.Results:1) CBDL group, rhGH group than in the SO group compared, TBIL, DBIL, ALT levels were significantly increased (P<0.05); TBIL, DBIL, ALT levels were significantly higher compared with rhGH group CBDL group, there are differences (P<0.05); CBDL14day group than CBDL7day group increased liver function levels were statistically significant (P<0.05); rhGH group was not statistically significant with time (P>0.05).2) CBDL group, rhGH group than in the SO group compared to the serious injury to the intestinal mucosa, a significant difference (P<0.05); CBDL group with obstructive intestinal mucosal injury over time progressively increased (P<0.05); rhGH group CBDL group compared with the respective periods decreased intestinal mucosal injury, there is a significant statistical significance (P<0.05); rhGH group was not statistically significant over time (P>0.05).3) CBDL group, rhGH group than in the SO group compared to the intestinal mucosa tight junction claudin-3protein levels were significantly reduced, there is statistically significant (P<0.05); CBDL group than rhGH expression of claudin-3group sessions levels were significantly lower, with the difference (P<0.05); rhGH group delay time with the expression level of claudin-3slightly increased, but no significant difference (P>0.05); CBDL group over time claudin reduce-3expression levels were statistically significant (P<0.05).4) CBDL group, rhGH group than in the SO group compared to the intestinal mucosa tight junction protein expression levels were significantly increased PKA have statistically significant (P<0.05); CBDL group than in the respective period of rhGH group PKA expression levels were significantly increased, there are differences (P<0.05); rhGH group with a delay time, the level of expression of PKA is slightly reduced, but no significant difference (P>0.05); CBDL groups with PKA expression level increased over time, there statistically significant (P<0.05).Conclusion:1. rhGH can increase the intestinal mucosa of rats with obstructive jaundice tight junction protein claudin-3expression.2.Obstructive jaundice rats pathway may damage the intestinal mucosal barrier function by PKA signaling. |
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