Study On The Function Of Mitochondrion Respiratory Chain Module Of Deficiency-cold Syndrome And Deficiency-heat Syndrome In The Different Environment Temperature

Objective: In order to explore the energy metabolism mechanism of the syndromefeatures of deficiency-cold syndrome and deficiency-heat syndrome, and reveal itsinternal molecular principle. The function of mitochondrion respiratory chain modulewere detected for the deficiency-cold syndrome and deficiency-heat syndrome in thedifferent environment temperature, Which lay a theoretical foundation for revealingthe internal principle of deficiency-cold syndrome and deficiency-heat syndrome at thelevel of molecular level.Method: First, tipical subjects were selected and divided into three groups with20members of a group: normal control group, deficiency-cold syndrome group anddeficiency-heat syndrome group according to the questionnaire of CCMQ and expertdiagnosis. All of them come from the undergraduate of grade1-3in Henan college oftraditional Chinese medicine in November2012. And then they were arranged intocold temperature room (about-4℃-5℃)30minutes and normal temperature room(23℃-25℃)20minutes. Finally their venous blood were collected to evaluate theEnzyme activity of respiratory chain of mitochondrion and mitochondrial membranepotential by spectrophotometry and FCM respectively.Results:1The variety law of respiratory chain complex Ⅰof mitochondrion:○1Betweendifferent syndromes: at the two different ambient temperatures, the activity ofcomplexⅠof mitochondrial was different in various syndrome group, which allshowed that deficiency-heat syndrome group﹥normal control group﹥deficiency-coldsyndrome group. Compared with the normal control group at the cold temperature, theactivity value of deficiency-cold syndrome group had significant difference(P<0.05)while there were no significant difference in deficiency-heat syndromegroup(P>0.05),there were significant difference between deficiency-cold syndromeand deficiency-heat syndrome (P<0.05).the result of room temperature consistent withthe result of cold temperature.○2The same group in different temperatures: compared with room temperature, the value of complex Ⅰof this three groups all rose at coldtemperature, and the value of complex Ⅰonly had significant difference(P<0.05) innormal control group, and there were no significant difference(P>0.05)indeficiency-heat syndrome group and deficiency-cold syndrome group.○3The variationof the adjustment rate of complex Ⅰ: compared with the normal control group at thecold temperature, the adjustment rate of deficiency-heat syndrome group anddeficiency-cold syndrome group all reduced with no significant difference(P>0.05),and there were also no significant difference between them(P>0.05), the result of roomtemperature consistent with the result of cold temperature; At cold temperature,compared with the room temperature, the adjustment rate of the three groups all rose,and only the normal control group had significant difference(P<0.05), and there wereno significant difference(P>0.05)in deficiency-heat syndrome group anddeficiency-cold syndrome group.2The variety law of respiratory chain complex Ⅱ of mitochondrion:○1Betweendifferent syndromes: at the two different ambient temperatures, the activity of complexⅡ of mitochondrial was different in various syndrome group, which all showed thatdeficiency-heat syndrome group﹥normal control group﹥deficiency-cold syndromegroup. Compared with the normal control group at the cold temperature, the activityvalue of deficiency-cold syndrome group had significant difference(P<0.05) whilethere were no significant difference in deficiency-heat syndrome group(P>0.05),therewere significant difference between deficiency-cold syndrome and deficiency-heatsyndrome (P<0.05).the result of room temperature consistent with the result of coldtemperature.○2The same group in different temperatures: compared with roomtemperature, the value of complex Ⅱ of this three groups all rose at cold temperature,and the value of complex Ⅱ only had significant difference(P<0.05) in normal controlgroup, and there were no significant difference(P>0.05)in deficiency-heat syndromegroup and deficiency-cold syndrome group.○3The variation of the adjustment rate ofcomplex Ⅱ: compared with the normal control group at the cold temperature, theadjustment rate of deficiency-heat syndrome group and deficiency-cold syndromegroup all reduced with significant difference(P<0.05), and there were no significantdifference between them(P>0.05), the result of room temperature consistent with theresult of cold temperature; At cold temperature, compared with the room temperature,the adjustment rate of the three groups all rose, and the normal control group and thedeficiency-heat syndrome group all had significant difference(P<0.05) while there were no significant difference(P>0.05)in deficiency-cold syndrome group.3The variety law of respiratory chain complex Ⅲ of mitochondrion:○1Betweendifferent syndromes: at the two different ambient temperatures, the activity of complexⅢ of mitochondrial was different in various syndrome group, which all showed thatdeficiency-heat syndrome group﹥normal control group﹥deficiency-cold syndromegroup. Compared with the normal control group at the cold temperature, the activityvalue had no significant difference in deficiency-heat syndrome group anddeficiency-cold syndrome group (P>0.05), there were significant difference betweendeficiency-cold syndrome and deficiency-heat syndrome (P<0.05). the result of roomtemperature consistent with the result of cold temperature.○2The same group indifferent temperatures: compared with room temperature, the value of complex Ⅲ ofthis three groups all rose at cold temperature with significant difference (P<0.05).○3The variation of the adjustment rate of complex Ⅲ: compared with the normal controlgroup at the cold temperature, the adjustment rate of deficiency-heat syndrome groupand deficiency-cold syndrome group all reduced with no significant difference(P>0.05),and there were also no significant difference between them(P>0.05), the result of roomtemperature consistent with the result of cold temperature; At cold temperature,compared with the room temperature, the adjustment rate of the three groups all rose,and there were significant difference(P<0.05)in these three groups.4The variety law of respiratory chain complex Ⅳ of mitochondrion:○1Betweendifferent syndromes: at the two different ambient temperatures, the activity of complexⅣ of mitochondrial was different in various syndrome group, which all showed thatdeficiency-heat syndrome group﹥normal control group﹥deficiency-cold syndromegroup. Compared with the normal control group at the cold temperature, the activityvalue of deficiency-cold syndrome group had significant difference(P<0.05) whilethere were no significant difference in deficiency-heat syndrome group(P>0.05),therewere significant difference between deficiency-cold syndrome and deficiency-heatsyndrome (P<0.05).the result of room temperature consistent with the result of coldtemperature.○2The same group in different temperatures: compared with roomtemperature, the value of complex Ⅳ of this three groups all rose at cold temperature,and the value of complex Ⅳ had significant difference(P<0.05) in normal controlgroup and deficiency-heat syndrome group, there were no significantdifference(P>0.05)in deficiency-cold syndrome group.○3The variation of theadjustment rate of complex Ⅳ: compared with the normal control group at the cold temperature, the adjustment rate of deficiency-heat syndrome group anddeficiency-cold syndrome group all reduced,with the deficiency-cold syndrome grouphas significant difference(P<0.05)while deficiency-heat syndrome group has nosignificant difference(P>0.05), and there were also no significant difference betweenthem(P>0.05). at the room temperature, the adjustment rate of deficiency-heatsyndrome group and deficiency-cold syndrome group all reduced with significantdifference(P<0.05) and there were also significant difference between them(P<0.05);At cold temperature, compared with the room temperature, the adjustment rate of thethree groups all rose, and there were significant difference(P<0.05)in normal controlgroup and deficiency-heat syndrome group while there were no significantdifference(P>0.05)in deficiency-cold syndrome group.5The variety law of respiratory chain complex Ⅴ of mitochondrion:○1Betweendifferent syndromes: at the two different ambient temperatures, the activity of complexⅤ of mitochondrial was different in various syndrome group, which all showed thatdeficiency-heat syndrome group﹥normal control group﹥deficiency-cold syndromegroup. Compared with the normal control group at the cold temperature, the activityvalue had no significant difference in deficiency-heat syndrome group anddeficiency-cold syndrome group (P>0.05), and there were also no significantdifference between deficiency-cold syndrome and deficiency-heat syndrome(P>0.05).the result of room temperature consistent with the result of cold temperature.○2The same group in different temperatures: compared with room temperature, thevalue of complex Ⅴ of this three groups all rose at cold temperature with significantdifference (P<0.05).○3The variation of the adjustment rate of complex Ⅴ: comparedwith the normal control group at the cold temperature, the adjustment rate ofdeficiency-heat syndrome group and deficiency-cold syndrome group all reduced withno significant difference(P>0.05), and there were also no significant differencebetween them(P>0.05), the result of room temperature consistent with the result ofcold temperature; At cold temperature, compared with the room temperature, theadjustment rate of the three groups all rose, and there were significantdifference(P<0.05)in these three groups.6the variety law of MMP:○1At the two different ambient temperatures, theMMP was different in various syndrome group, which all showed that normal controlgroup﹥deficiency-heat syndrome group﹥deficiency-cold syndrome group. Comparedwith the normal control group at the cold temperature, the MMP had significant difference in deficiency-heat syndrome group and deficiency-cold syndrome group(P<0.05), and there were no significant difference between them (P>0.05); Comparedwith the normal control group at the room temperature, the MMP had no significantdifference in deficiency-heat syndrome group and deficiency-cold syndrome group(P>0.05), and there were also no significant difference between them (P>0.05)○2Compared with room temperature, the MMP of the normal control group anddeficiency-heat syndrome group all rose at cold temperature, and the value of thenormal control group had significant difference(P<0.05)while there were no significantdifference(P>0.05)in deficiency-cold syndrome group. Although the MMP ofdeficiency-cold syndrome group in cold temperature were above room temperature, buta had no significant difference(P>0.05).Conclusion:All mitochondrial respiratory transport chain complexes and MMP were abnormalin the deficiency-cold syndrome and deficiency-heat syndrome, and when thetemperature changed, there were deficiencies in the enzymatic activity of theadjustment ability of deficiency-cold syndrome and deficiency-heat syndrome, whichmaybe the material basis of deficiency-cold syndrome and deficiency-heat syndrome, itneeded to do more research on it to know more.