Study On Anti-benign Prostatic Hyperplasia Active Ingredients Of Rape Bee Pollen

Rape bee pollen is the aggregate object from canola anther collected by bees and addedglandular secretions of bees. It has good anti-aging, improve immunity, anti-prostatehyperplasia, anti-tumor and many other physiological activities. Rape bee pollen has excellentanti-benign prostatic hyperplasia activity, but the active ingredients and mechanism are notclear until now. In this paper, we use rape bee pollen as research object and study its activeingredients of anti-BPH.Rape bee pollen extracted by90%ethanol, then extracted by different organic solventsone by one, we get four parts as petroleum ether extract, ethyl acetate extract, n-butanolextract and the raffinate. Study the anti-BPH activity of each pollen extract with high (400mg/kg·d), medium (200mg/kg·d) and low (100mg/kg·d) dose by the androgen-induced micemodel of BPH. The results show that the high, medium and low dose of ethyl acetate extractand the high dose of petroleum ether extract could significantly reduce prostate index, contentof DHT, T, E2, activity of PAP of model mice (P <0.05or P <0.01); the high and mediumdose of ethyl acetate extract and the high dose of petroleum ether extract could obviouslyreduce prostate weight (P <0.05). So we can conclude that the extract of ethyl acetate andpetroleum ether have significant activity of anti-BPH.We get rough extracts PI, PII, PIII separated from the extract of ethyl acetate andpetroleum ether by MCI chromatographic column. Study the anti-BPH activity of each roughextract with high (200mg/kg·d), medium (100mg/kg·d) and low (50mg/kg·d) dose by theandrogen-induced mice model of BPH. The results show that the high, medium and low doseof PII and the high and medium dose of PIII could significantly reduce prostate index, contentof DHT, T, E2, activity of PAP of model mice (P <0.05or P <0.01); the high and mediumdose of PII and the high dose of PIII could obviously reduce prostate weight (P <0.05). Sowe can conclude that the rough extract PII and PIII have significant activity of anti-BPH.We get compound1from PI, compound2,3and4from PII, compound5and6fromPIII separated by MCI and ODS chromatographic column. We identificate the structures fromNMR spectra, compound1, Kaempferol-3-O-β-D glucopyranoside; compound2, Octadecene-9Z,12Z,15Z-three acid glycerides; compound3,3’-methoxy-1’-octadecene-9Z,12Z,15Z-three acid glycerides; compound4, Octadecene-9Z,12Z,15Z-trienoyl-N-ethylglyceryl ether; compound5,2’-methoxy-1’,3’-octadecene-9Z,12Z,15Z-three aciddiglyceride; compound6, α-linolenic acid.Study the anti-BPH activity of each compound with high (100mg/kg·d) and low (50mg/kg·d) dose and mixture (2+3) with [(50+50) mg/kg·d] dose by the androgen-induced micemodel of BPH. The results show that the high and low dose of compound3, the high dose ofcompound2and mixture (2+3) significantly reduce prostate weight, index, content of DHT, T,E2, activity of PAP of model mice (P <0.05or P <0.01). So we can conclude that thecompound2,3and mixture (2+3) have significant activity of anti-BPH. We also can find thatcompound2and compound3have obvious synergies in the statistical data.