Induced Differentiation Of Adipose-derived Stem Cells For Tissue Engineering Blood Vessel

Now vascular grafting is a frequent and important surgical procedure to cure vascular diseases in the clinical medicine. Internal mammary artery or autologous saphenous vein are usually used as material.But autologousvessel that can be available for transplantation is limited, and a large part of patients are not suitable for autologous vascular transplantation. Artificial blood vessels such as Dacron and expanded polytetrafluoroethylene has been successful in large diameter (inner diameter>5mm) vasculartransplantation, but in small diameter (inner diameter<5mm)vascular transplantation due to its high shear stress and low blood flow which can lead to thrombosis and neointimal hyperplasia, the long-term patency rate is not ideal. At the same time, the lack of degradability and promoting tissue regeneration capacity, long-term transplantation in the presence of poor biocompatibility issues are limiting factors for clinicl application. The development of tissue engineering for the study of biodegradable, biocompatible of small diameter vascular scaffolds provides a new approach for the source of vascular grafts.Adipose-derived stem cells and MSC has similar differentiation potential in vitro, both of them can differentiate into a variety of mesoderm-derived cells, such as osteoblasts, chondrocytes, myocytes. Adipose-derived stem cells can be easily obtained by liposuction, little trauma and adequate supply. And adipose stem cells have the advantage of the rapid proliferation. What,s more,it dose not have moral argument.So there will be beoad prospect in the application of adipose-derived stem cells to vascular tissue engineering.In this study, we exploit the multilineage differentiation of adipose stem cells,by in situ transfection inducing ADSC to the vascular endothelial cells and vascular smooth muscle cell. We make of tissue engineering scaffolds using gelatin, PEI as transfection carrier. This system has high transfection efficiency. VEGF activated scaffold can induce adipose-derived stem cells (ADSC) differentiation into vascular endothelial cells; PDGF can induce adipose-derived stem cells (ADSC) to vascular smooth muscle cells.. Vascular endothelial cells and vascular smooth muscle cell potential of ADSC in vitro was assessed using scanning electron micrograph, ELISA assay, Q-PCR assay.Gene activated material is able to support the attachment and proliferation of ADSC by the result of cell count; ELISA assay shows the expression amount of VEGF and PDGF. The result indicates that the scaffold is an ideal in situ transfection system. Q-PCR results show ADSCs are induced to express vascular endothelial cell marker CD31and vWF. In the same way, ADSCs different to vascular smooth muscle. Compared to non-gene activated system, mRNA levels of cnnl and alpha-SMA for vascular smooth muscle cells markers are significantly up-regulated.This study shows, ADSC can different into vascular endothelial cells and vascular smooth muscle cells under the suitable induction conditions; ADSC can be provided as seed cells for vascular tissue engineering vessel research.