Isolation And Culture Methods Of Rabbit Adipose-Derived Stem Cells

Objective To establish a simple and effective method for isolation and culture of rabbit adiposen-derived stem cells (ascs) in vitro, in order to provide the sufficient sources of seed cells for the research of mesench ymal stem cells.Mechods The Methods of rabbit inguinal fat tissue separation and perform with 0.1% collagenase digestion in vitro type, then adipose-deri vedstem cells (ascs) were seeded, adhere to The culture dish to low gluco se DMEM containing 10% fetal bovine serum. The cell morphology, proli feration in vitro, multidifferentiation potential and immunophenotype assess ed.Results The ASCs showed good cell morphology, fat to form stron g proliferation ability and potential, in vitro osteogenesis and cartilage for mation of three-dimensional induction. The cell surface antigen phenotype are consistent with The literature, and The expression of CD34 and CD 10 5 was positive Sca-1 was highly expressed, CD45 and SSEA-1 were not expressed.Conclusion Using rabbit inguinal hypodermic and adipose tissue, can produce high purity, has a good stem cell characteristics and strong p roliferation ability of adipose stem cells in rabbits.