The Significance And Relationship Among The Matrix Metalloproteinase-2, Transforming Growth Factor-β1and Cystatin C In Patients With Chronic Hepatitis B And Cirrhosis

Objective：In this study of matrix metalloproteinase-2(MMP-2), transforminggrowth factor-β1(TGF-β1) and cystatin-C (Cys-C) and hepatic fibrosis index with theseverity of liver disease. Analysis the correlation of Cys-C, MMP-2, TGF-β1for furtherstudy the mechanisms of liver fibrosis, provide a new method for the early diagnosis andprevention of hepatic fibrosis.Methods：13patients with chronic hepatitis B(CHB) and14post-hepatitis B livercirrhosis （PHBC）in the People’s Hospital of Liaoning Province were recruited todetermine their serum levels of MMP-2、TGF-β1and Cystatin C, together with otherhepatic parameters of liver fibrosis of hyaluronic acid and procollagen Ⅲ. These werecompared with10normal controls. Collection of blood through the veins，centrifugingthem5minutes at3000r/min on the centrifuge. Indwelling serum in EP tube. Numberedand marked the sample respectively. Before testing, serum was stored at-70℃refrigerator.Determination the levels of serum MMP-2, TGF-β1and hepatic fibrosis index byenzyme-linked immune sorbent assay (ELISA). Collect all the selected objects lab Cys-C test results. Then analyze the relevant indicators.Result: Using SPSS19.0statistical software for data analysis, application analysisof variance analysis method MMP-2, TGF-β1, Cys-C, HA, PC Ⅲ in the differencesbetween the groups. Using Pearson correlation analysis between these indicators. Eachset of data in line with normal distribution, the basic situation such as age, sex and creatinine levels between experimental groups had no significant statistical difference.TGF-β1and MMP-2in CHB and PHBC group is significantly higher than the controlgroup (P<0.05), MMP-2and TGF-β1PHBC group is significantlyhigher than in patientswith CHB patients (P<0.05), there are significant differences between the groups. Cys-Cin the CHB Group and the control group no significant statistical significance (P>0.05),in the PHBC and control groups had statistically significantly (P<0.05), between PHBCand CHB groups there are also distinct statistical significance (P<0.05). HA and PCⅢlevels in liver disease was significantly higher. Through the Pearson correlation analysis,MMP-2, TGF-β1, Cys-C significantly correlated with the HA, PCⅢ (MMP-2=0.643+0.003HA,R=0.555; MMP-2=0.824+0.016PC,R=0.326;TGF-β1=9.233+0.357HA,R=0.587;TGF-β1=7.506+3.393PC,R=0.652;Cys-C=0.179+0.006HA,R=0.764;CysC=0.415+0.034PCⅢ,R=0.546).Cys-C with MMP-2and TGF-β1have significantl-y correlated（Cys-C=0.177+0.686MMP-2,R=0.530;Cys-C=0.541+0.006TGF-β1,R=0.533）. TGF-β1andMMP-2exist significant correlation（TGF-β1=2.861+49.621MMP-2,R=0.463）.Conclusion:①Through this study, we concluded that with advances in chronicviral hepatitis and cirrhosis, serum levels of MMP-2, TGF-β1andCys-C on a rising trend,and there is a correlation with hepatic fibrosis.②Cys-C in the process of hepatic fibrosisthere might be some linkages betweenMMP-2and TGF-β1.③By detecting serum MMP-2, TGF-β1, Cys-C in conjunction with other serological and imaging tests, it is better forearly diagnosis of hepatic fibrosis in liver diseases and early intervention. Reduce theincidence of liver cirrhosis and liver cancer, and improve the long-term prognosis.