| BackgroundDiabetic retinopathy is a microvascular complication of diabetes mellitus, the pathogenesis is complex and diverse. But at the onset of different DR and different pathogenesis theory, the common is that there has increased the concentration of oxygen free radicals, accompanied by reduced antioxidant substances. Edaravone is a powerful antioxidant with a strong free radical scavenging capacity, which can protect the tissue against oxidative stress damage. Therefore, to study the intervention effect of edaravone on DR, not only helps to further clarify the pathogenesis of DR, but also contributes to a better DR control.ObjectiveTo explore the effect and significance of edaravone on retinal oxidative stress in diabetic rats.Methods1. The object of this study is30male SD rats. These rats were divided into3groups:10rats with normal blood sugar as control group,10rats with high blood sugar as diabetic model group,10diabetic rats treated by edaravone as the edaravone treatment group.2. The total protein of retina in rats of each group was extracted by cell lysate, the SOD activity, GSH activity and the content of MDA in the retinas were detected by SOD Activity Kit, GSH Activity Kit, and MDA Kit respectively.3. The differences of GFAP protein expression in the retinas were detected by the application of Western-Blot technology, the expression of β-actin protein was used as a control.Results1. Compared with normal blood sugar control rats, diabetic rats showed decreased SOD activity, GSH activity and increased MDA content in the retina. While edaravone can increase the activity of SOD and GSH in the retina of diabetic rats and decrease the production of MDA.2Compared with normal blood sugar control rats, diabetic rats showed significant higher retinal GFAP protein content and the protective effect of edaravone can reduce the expression of GFAP.Conclusions1. Compared with the normal control group, diabetic rats retinal SOD activity, GSH activity were decreased, while the MDA compared with the normal control group were increased, suggesting that increased levels of oxidative stress in diabetic retina. Edaravone can inhibit the decline of SOD activity, GSH activity, and decrease the production of MDA, so as to reduce oxidative stress injury of retinal function, resulting in the decreased expression of GFAP in the retina of diabetic rats.2. Antioxidative effects of edaravone suggests that edaravone can be a drug of early diabetic retinopathy intervention, but its effectiveness remains to be further studied. |
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