Association Study Between NR3C2Gene Polymorphism And Aggressive Behaviour

Objective:Attack is the behavior that the purpose of any form of harm to another living organism who rather reluctant to accept.The mechanism of aggressive behavior is becoming today’s research focus, however, due to the complexity of of human behavior and the limitations of research methods, mechanisms of aggressive behavior has not been elucidated, many studies suggest that violent attacks in addition to related to social, psychological factors, and the central neurotransmitter and its related genes and gene expression has close relationship.This topic chooses initial trigger receptors involved in stress response-mineralocorticoid receptor gene (Nuclear receptor subfamily3, group C, member2, NR3C2) as candidate genes were analyzed, to explore the correlation NR3C2gene polymorphism and violent aggressive behavior,to clarify the mechanism of aggressive behavior and the use of gene prediction of violent attacks occurred, even to fight violent attacks designed to provide scientific basis for drug targets. Method:This paper using saturated phenol-chloroform extraction DNA and the improved multi-temperature ligase detection reaction technique (iMLDR) technology119cases of violent attacks prison inmates and136cases of healthy control individuals NR3C2gene4SNPs points (rs2070951, rs5522, rs5525, rs2871) were genotyped analysis, Perform statistical analyses using softwares like SPSS17.0, Haploview, and SHEsis.Results:(1) The result of single locus analysis:rs2070951, rs2871, rs5522and rs5525locus genotyping were the Hardy-Weinberg equilibrium (p>0.05). rs2070951C and G allele frequency differences among distributed in violent attacks and control groups were statistically significant (χ2=5.296, P=0.021, OR=1.683). rs2070951genotypes(CC,CG and GG)frequency distribution difference in two groups have statistical significance (χ2=5.139, P=0.077).rs2070951loci of wild type CC and mutant (CG+GG) distribution difference in two groups have statistical significance (χ2=5.020, P=0.025, OR=1.797). rs5522C and T allele frequency differences among distributed in violent attacks and control groups were statistically significant(x2=5.146, P=0.023,OR=1.957);the frequency distribution of rs5522locus genotype (TT,CT and CC)(χ2=4.795, P=0.091)differences between the two groups were no statistically significant; wild-type TT and mutant (CT+CC) frequency distribution (χ2=3.786, P=0.052) differences between the two groups were no statistically significant.rs5525A and G allele frequency differences among distributed in violent attacks and control groups were statistically significant(x2=5.146, P=0.023,OR=1.957); the frequency distribution of rs5525locus genotype (AA,AG and GG)(χ2=4.795, P=0.091differences in the two groups were no statistically significant; wild-type GG and mutant (AT+AA) frequency distribution (χ2=3.786, P=0.052) differences in the two groups were no statistically significant.rs2871C and T allele frequency distribution (χ2=0.297, P=0.586) and frequency distribution of genotypes (χ2=1.307, P=0.520) in the two groups were not statistically significant.wild-type TT and mutant (CT+CC) frequency distribution (χ2=0.682,P=0.409) differences in the two groups were no statistically significant.(2)The result of linkage disequilibrium analysis:In the violent attack group, the control group and both of the two groups conducting linkage disequilibrium analysis, D’values of rs2070951-rs5522, rs2070951-rs5525and rs5522-rs5525were greater than0.8; rs2070951, rs5525and rs5522were in the same linkage disequilibrium area, and there is a strong linkage disequilibrium.(3) The result of haplotype analysis:Haplotypes of rs2070951-rs5522, rs2070951-rs5525, rs5525-rs5522and rs5525-rs2871exist significant differences in the distribution between the group of violent attacks and the control group from overall test results (P values were less than0.05). logistic regression analysis showed that haplotype of rs5525-rs5522A-C(χ2=5.146,P=0.023,OR=1.957)and G-T (χ2=5.146,P=0.023, OR=0.511) exist significant differences in the distribution between the group of violent attacks and the control group.(4)The results of interaction of the four NR3C2gene loci:This research adopts the MDR method to analyze the interaction of the four SNPs of NR3C2genes finding that the interaction between four SNPs loci best model does not exist.Conclution:(1) Rs2070951may be associated with violent attacks. the person carrying mutant allele G orgenotype (CG+GG) significantly increased the risk of violence against the behavior.(2)Rs5522loci of the allele frequency distribution may be associated with violent attacks, carrying mutant allele C significantly increased the risk of violence against the behavior.(3)Rs5525loci of the allele frequency distribution may be associated with violent attacks, carrying mutant allele A significantly increased the risk of violence against the behavior.(4)Rs2871sites may have nothing to do with violent attacks.(5)haplotypes of rs2070951-rs5522, rs2070951-rs5525, rs5525-rs5522and rs5525-rs2871may be associated with violent attacks. Haplotype of rs5525-rs5522A-C significantly increased risk of aggressive behavior of human violence,Haplotype G-T makes the risk of human violence aggressive behavior significantly reduce.