Part â…  Metabolic Status In Diabetes Families In Yunnan Province

[Objective] To understand the Clinical features and the metabolic status in type2diabetes mellitus of han population families in Yunnan province. And compare the difference between familial and non-familial type2diabetic population.[Methods] Collected203cases of familial type2diabetes,192cases of non-familial patients with type2diabetes,175cases of healthy controls, compare of general clinical features, blood glucose, blood lipids, HbAIc, lactic acid, insulin-C peptide and proinsulin and true insulin levels in the three groups. Evaluated islet beta cell’function and insulin resistance.[Results] There was a significant difference between the familial type2diabetes mellitus group and normal control group in AGE, SBP, WHR, FPG, PPG, HbA1c, DBP, FINS, BMI, HDL-C, PINS, ISI, HOMA-IR, PI, TI, FCP, PCP, HOMA-β. And there was a significant difference between the no-familial type2diabetes mellitus group and normal control group in AGE,SBP,WHR,FPG,PPG,TG,HDL-C,LDL-C. And there was a significant difference between the familial type2diabetes mellitus group and the no-familial type2diabetes mellitus group in PPG,ISI,HDL-C,FINS,PrNS,HbA1c,HOMA-IR,PI,TI,LDL-C.[Conclusion]1.True insulin may participate in the onset of familial type2diabetes.2.High density lipoprotein is an independent protection factor of the familial diabetes. [Objective] To explore the distribution and clinical features of various mitochondrial(mt)DNA ND1gene mutations, ND4gene mutations and D-loops gene mutations among the familial diabetes mellitus of Chinese in Yunnan Province. In order to find new pathogenic sites in MDM.[Methods] PCR restriction fragment length polymorphism (PCR-RFLP) analysis was used to screen point mutations of mtDNA (3426,12026,16189) in203cases Familial type2diabetes,192cases of non-kinship in patients with type2diabetes and175healthy controls, followed by direct sequencing to confirm mutation。[Results] There were4carries (1.97%) of12026A→G mutations,66carries (32.5%) of16189T→C mutation in the experimental group; there were10carries (5.21%) of12026A→G mutation,60carries (31.25%) of16189T→C mutation in the non familial T2DM group, there were7carries (4.0%) of12026A→Gmutations, there were18carries (10.3%) of16189T→C mutations in the normal glucose tolerance group.Of all the tested objects were not detected3426A→Gmutation. Each mutation point sequencing results are consistent with the PCR-RFLP patterns.MtDNA12026locus mutation rate in each group differences among groups was not statistically significant (P>0.05). The mtDNA16189locus mutation rate between familial T2DM group and non-familial T2DM group was not statistically significant (P>0.05), and the mtDNA16189locus mutation rate between familial T2DM group and the normal glucose tolerance group was statistically significant (P=0.000<0.05), and the mtDNA16189locus mutation rate between non-familial T2DM group and the normal glucose tolerance group was statistically significant (P=0.000<0.05).[Conclusion]1.It’s shown that the frequency of mtDNA3426A→Gmutation is fairly low of patients with T2DM in Yunnan area.2. mtDNA12026A→G mutations is not associated with the family history of maternally inherited diabetes mellitus,12026A→G mutation may participate in the development of type2diabetes.3. Mitochondrial DNA16189T→C is associated with type2diabetes and insulin resistance. [Objective] To explore the mitochondrial gene mutations of Diabetes pedigrees, and analyze the genetic pattern and the clinical features of Mitochondrial DNA mutation sites in Diabetic pedigrees.[Methods] The patients with mtDNA16189T→C mutation and without mtDNA16189T→C mutation are the main research objects of this part of the study.According to the diagnostic criteria of Mitochondrial Diabetes Mellitus,we collect the families of the main research objects. We divided into the matrilineal inheritance diabetes families and the no-matrilineal inheritance diabetes families,and we compared two groups of mtDNA16189T→C mutation rate. We dixided into two groups:one is of C group that all family members are mtDNA16189T→C mutation positive (namely mutation rate was100%);the other one is of T group that all family members are mtDNA16189T→C mutation negative (namely mutation rate was0%).Compare various clinical indicators of two groups.[Results] The mtDNA16189T→C mutation rate of the matrilineal inheritance diabetes is65.79%(25/38), and the mtDNA16189T-+C mutation rate of the the no-matrilineal inheritance diabetes families is16.13%(5/31), the variation of the chi-square test show the mtDNA16189locus mutation rate between the matrilineal inheritance diabetes group and the no-matrilineal inheritance diabetes families group was statistically significant (P=0.001<0.05). There was a difference between all family members are mtDNA16189T→C mutation positive group and all family members are mtDNA16189T→C mutation negative group in WHR, PI, TI, HbAlc.[Conclusion]1.MtDNA16189T→C mutation is associated with maternal genetic type2diabetes.2. The matrilineal inheritance diabetes family with mtDNA16189T→C mutation positive have more severe degree of insulin resistance and the increased risk of Type2diabetes.