Preparation And Evaluation Of Molecularly Imprinted Monolithic Column Based On Thermoresponsive And Deep Eutectic Solvents

Molecularly imprinted polymers (MIPs) are a new technique for synthesizing the stationary phase with the ability of pre-selectivity and monolithic column which is a new type of chromatographic stationary phases. The combination of monolithic column and MIPs will be the chromatographic stationary phases with potential applications and played an important role in the area of analytical chemistry. Development of new type has good molecular recognition and high column efficiency of molecularly imprinted monolithic column is a important challenge of current research.Synthesised of imprinted monolithic columns with different methods, and different synthesize conditions, chromatographic conditions and the characteristic of MIP columns are systematically investigated. The results as follows:1In this work, an imprinted monolithic column is synthesized that using a mixture of ketoprofen (template), AAm, AMPS (functional monomer) by in-situ polymerization. Some polymerization factors, such as template-monomer molar ratio, the compositions between the functional monomer, on the imprinting effects of resulting MIP monolith are systematically investigated. The results are indicated that temperature change for ketoprofen and structural analogues of reservation and the recognition performance has a great influence, when the temperature is35degrees, the retention factors (k=0.242) of template is bigger than the retention factor of room temperature, then the imprinting factor was5.38.2A new approach based on deep eutectic solvents-choline chloride as porogen for synthesis of molecularly imprinted monolithic. An imprinted monolithic column is synthesized using a mixture of cetirizine (CET, template),4-vinylpyridine (4-VP), ethylene glycol dimethacrylate (EDMA),2,2’-Azobis (2-isobutyrontrile)(AIBN), cobalt acetate, choline chloride-ethylene glycol (Ach-EG), dimethyl formamide (DMF) and [BMIM]BF4. The influence of polymerization parameters such as the ratio of template to functional monomer, the degree of crosslinking on permeability, morpholophy of CET-imprinted monolith are systematically investigated. The results are illustrated that molecularly imprinted polymer exhibited excellent retention capability and large imprinted factor when the ratio of monomer and crosslinking agent is1:3(the retention factor and imprinted factor for cetirzine on MIP column were30.2and31.5, respectively).