Artificial Facet Joint With C/C-SiC Composites Biocompatibility And Mechanical Properties

Objective:To evaluate the C/biocompatibility and mechanical properties of C-SiC composites, as a preliminary to explore the feasibility of artificial facet joint transition coating material.Methods:1.cytotoxicity test:The ratio of materials of different extracts of mouse embryonic fibroblasts with a mixed culture, and set the negative control group and the positive control group to calculate the added value of relative cell observation of living cells by MTT assay and reference six toxicity rating criteria for toxicity assessment;2. micronucleus test:18Kunming mice were randomly divided into experimental group, negative control group and positive control group, n=6(male and female), the material extraction solution to50ml/kg dose injected into the peritoneal cavity of mice after four femur bone marrow toxicity stained smear, detected by micronucleus and polychromatic stained positive genotoxicity and assessment materials dyed red cell ratio;3.hemolysis test:rabbit anti-coagulant were added to the negative control group, positive control group and the test solution extract material and disposed in each group was visually observed hemolysis tube, and measure the absorbance of the supernatant in the centrifuge tube calculate the rate of hemolysis, caused by hemolytic evaluation materials;4.sensitization test:use18albino guinea pigs were randomly divided into experimental group, negative control group and positive control group6(male and female), after induction-excitation stage, observed after removing the applicator pieces24h,48h stimulate areas of skin reactions, evaluation materials sensitization.5.compressive, flexural and friction and wear tests:the C/C-SiC composites with C/C composites compression test on an electronic universal testing machine, bending performance, on the friction and wear tester measured friction and wear properties.Results:1.cytotoxicity test:Material extracts of mouse embryonic fibroblasts into morphology had no effect, with the extension of the growth period, the number of cellular material extract group even stronger than the negative group;2.micronuclei. test:mouse micronucleus test group was3.00%o, less than twice the value of the negative group, the material was not genotoxic;3.hemolysis test:the test group turbid liquid red, visible red blood cells deposited at the bottom of the tube, the supernatant after centrifugation was no color clarity shaped, measuring the absorbance of the supernatant material hemolysis value was0.521%<5%, material non-induced hemolytic;4.sensitization test:a test group has appeared a performance at24h, the rest are all normal, and48h all test groups all the normal skin, no allergic reaction performance;5.compressive, flexural and friction and wear tests:. C/C-SiC composites compared with C/C composites have better compressive, flexural and the friction and wear properties.Conclusion:C/C-SiC composites cell-toxic, non-genotoxic, non-allergenic, does not cause tissue hemolytic reaction, superior mechanical properties of compressive, flexural and friction wear, has good biocompatibility evaluation and mechanical properties, as a preliminary determination that has artificial facet joint transition coating material potential.