Effect Of Nourishing Kidney And Activating Blood Soup Which Prevents Kidney From Ischemia/Reperfusion Injury

Objective:To explore the protective effect of Nourishing Kidney and Activating Blood soup against renal ischemia reperfusion injury(RIRI) in mice.Methods:56male BALB/c mice (bw:20-25g)were randomly divided into Nourishing Kidney and Activating Blood soup treated group and control group,each group were divided into four groups:sham-operted group(SHAMc,SHAMn),ischemia/reperfusion5hours group(IR5c,IR5n),ischemia/reperfusion24hours group(IR24c,IR24n) and ischemia/reperfusion48hours group(IR48c,IR48n),with7mice in each group.Sham-operted groups only separated double kidneys and renal pedicles,while in ischemia/reperfusion groups local warm ischemia was induced by clamping both renal pedicles for35min,and then reperfused for5,24and48hours respectively. Collected blood and renal tissue to measure the level of serum creatinine(Scr), blood urea nitrogen (BUN)and observe the pathological changes of renal tissue under microscope, the tubular injury score was compared among each group.Results:Compared with SHAM groups, there were significantly higher level of Scr, BUN and higher score of tubular injury in I/R groups(P<0.05), and highest at24h after reperfusion. The level of Scr,BUN and the score of tubular injury in Nourishing Kidney and Activating Blood soup treated groups(IR5n, IR24n, IR48n)was significantly lower than that in control groups(IR5c, IR24c, IR48c)(P<0.05).Conclusion:l.The mice model of RIRI can be induced by clamping both renal pedicles for35min, and then reperfused for5,24and48hours respectively.2.Nourishing Kidney and Activating Blood soup was taken continuously before the model was induced could reduce the level of Scr,BUN and score of tubular injury,which prove that Nourishing Kidney and Activating Blood soup can protect kidney against RIRI in mice to some extent.3.Studies have shown that Astragalus, Schisandra, Salvia Miltiorrhiza and Ligustrazine can protect kidney against RIRI effectively,and its mechanism are related to the alleviation of inflammatory response, free radical injury, apoptosis,and change of renal tissue ultrastructure.It’s concluded that the mechanism of protective effect of Nourishing Kidney and Activating Blood soup on RIRI is related to multiple targeting effect of its main constituent.On the other hand,Nourishing Kidney and Activating Blood soup can protect RIRI by dilating renal vessels,improving renal blood flow and minicirculation and promoting the absorption of fibrous tissue.Its specific mechanism need for further study.