Pharmaceutical And Preliminary Pharmacodynamic Studies Arctiin Qingyan Lozenge

Objective: To study the preparation of Arctium Qingyan lozenges, establishingquality standards preparation, study its initial stability. And through preliminarypharmacodynamic study of Arctium Qingyan lozenges explore its pharmacologicalactivity of the treatment of acute laryngitis.Methods:①orthogonal experiment to extract volume as an indicator Paeonolpreferred moutan distillation process to extract the amount arctiin and chlorogenicacid as an indicator, decoction process preferably Arctiin Qingyan lozengesprescription Pieces; granulation process and the product of research, carried out ontablets flavoring screening experiments.②the preparation Appearance, weightvariation, disintegration, such as checks, while using thin layer chromatographyidentification Arctiin Qingyan tablet formulations Arctiin, honeysuckle and moutan,using high-performance liquid chromatography establish a method fordetermination of arctiin.③Arctiin preliminary stability study conducted byQingyan lozenges preclude the use of room temperature sample observation methodand accelerated testing.④by ear edema in mice, rats carboxymethyl cellulosepouch experiments, acetic acid-induced writhing test and affect body reactions dueto the hot plate pain threshold in mice and in vitro inhibition experiments to explorethe preparation the efficacy of acute pharyngitis.Results:①Arctium Qingyan lozenges best preparation process: Peony Piga12times the amount of water, distillation5h,6times distillate collected, filtereddecoction, spare; distilled liquid refrigerated for24hours, filtered, was Dan Papercrystalline phenol, cool, dry and set aside. The rest Arctiin and other flavors plus8times the amount of water, boiling three times, each time1h, filtration, combinedfiltrate and combined with the above decoction, evaporated to a relative density of1.025(60℃) liquid, centrifugation, continue evaporated into the relative density of1.25(60℃) of a thick paste, dried under reduced pressure, combined withpaeonol crushed to a fine powder, add powdered sugar, dextrin, citric acid, Ansaihoney, mix;0.25granulation times of80%ethanol, over a12mesh sieve, and dried. Adding magnesium stearate particles sprayed into ethanol dissolved menthol,moistening, tablet, that is.②quality standard: the establishment of Arctium TLC method: using silicagel G plate with dichloromethane-methanol-water (40:8:1) as eluent,10%sulfuric acid in ethanol as chromogenic reagent,105℃heating color.Honeysuckle TLC method: silica gel G plate to butyl acetate-formic acid-water(7:2.5:2.5) upper solution as the agent,105℃heating color. TLC methodmoutan: silica gel G plate with cyclohexane-ethyl acetate (3:1) as the agent,sprayed with hydrochloric acid5%ethanol solution of ferric chloride, UV light(365nm) to view the results. Developed using high-performance liquidchromatography method for the determination of arctiin, octadecylsilane bondedphase silica as a filler, methanol-water as the mobile phase, flow rate1.0mL min-1, detection wavelength280nm. Investigate the linear relationship: theregression equation was Y=482525X-83615, r=1.0000; linear range of0.586~5.274μg. Negative interference test results showed peaks with arctiin separatedfrom other components of the product is good, the negative interference. Theprecision RSD=0.8%, stability test RSD=1.5%, reproducibility test RSD=1.95%,recovery test average recoveries98.94%, RSD%=1.68.③Experimental results show that the stability of the indicators of the finishedproduct Appearance, identification, examination, and content of at acceleratedconditions did not change significantly at room temperature.④pharmacodynamic experiments show Arctiin Qingyan lozenges high dosegroup inhibited xylene-induced mouse ear inflammation swelling with increasingdose, and this inhibition is enhanced. Carboxymethylcellulose rat bladder resultsshowed carboxymethylcellulose inflammation after3h, the positive control drugaspirin inhibits leukocyte migration, and inhibition of the product in each dosegroup was not obvious; carboxymethyl after cellulose proinflammatory7.5h, thepreparation of high, medium dose group inhibits leukocyte migration, andincreased with dose, and this inhibition is enhanced. Hot plate induced painthreshold in mice experimental results show that the pain caused by the reaction ofthe hot plate Arctium Qingyan lozenges extended latency period. Afteradministration30min, high-dose group compared with the model group, P <0.01;after administration60min, high dose group compared with model group, P <0.01.Acetic acid-induced writhing response of experimental results show that the high -dose group Arctiin Qingyan lozenges may reduce mouse acetic acid-inducedwrithing, compared with the model group, P <0.01. Select the common pathogenscausing acute pharyngitis bacteriostatic experiment results show that the product ofStreptococcus pneumoniae, beta-hemolytic streptococcus, Escherichia coli,Staphylococcus aureus antibacterial tablets are better than pharyngitis, which ishighly sensitive Staphylococcus aureus.Conclusion:①The experimental results show that the preparation processArctiin Qingyan lozenges scientific and rational, sensitive and reliable methods ofquality standards and specific stronger. During storage at room temperature,acceleration and quality stable.②Arctiin Qingyan lozenges have goodanti-inflammatory, analgesic and antibacterial effects.