Study On Wu Ling-tishen Pian

The topics with guidance of Traditional Chinese Medicine theory and technology of modern pharmacy, According to the laws, regulations and methods of traditional Chinese medicine health food, developed a health care product of Wu Ling-tishen piece for relieve physical fatigue. It is consists of Ginseng, Ophiopogon, Schisandra and fungus as raw materials.The preparation according to the function of health care products, comparison of anti-fatigue function of water extract and alcohol extract, by exercise tolerance test, measurement of muscle glycogen and liver glycogen, blood lactate, blood urea, also verify the validity of the prescription anti-fatigue function. The results showed that the ethanol extract of the sample specimen is obviously anti-fatigue function than water extraction. Filter results based on a single factor, the extraction solvent and method of the prescription is75%ethanol percolation extraction. By percolation extraction and reflux extraction orthogonal test screening and optimization process as: medicinal herbs were crushed to a coarse powder, plus10times the amount of75%ethanol, dipping12h, speed with7mL·in-1·kg-1for percolation, collecting the filtrate, the filtrate was concentrated to a thick paste, and placed into60℃vacuum drying oven was dried with phosphorus pentoxide to obtain the extract was dried.In this study, according to the feature of prescription of extract power, tablets are determined as the prescription formulations. In the process of making, single factor screens out the types of filler and blinder,using Design Expert8.0mixture for the optimization of formulation to search for the best formula. Preparation of optimized preparation process is:extract68.8%, silica gel powder4.9%, starch26.3%and three components are mixed uniformly, adding concentration of20%PVPk30alcohol solution of binder, granulating, drying oven at60℃within1hours, making a whole grain and compression.Study on quality standard establishes the TLC method for the identification of five pieces of ginseng, Ophiopogon japonicus, Schisandra chinensis, Ganoderma lucidum. Inspections have been done with the tablet formulations of general rules including inspection of the weight difference, disintegration test, microbial inspection. The results are in line with the pharmacopoeia. The system method for determination of schisandrin in the prescription of anti fatigue functional components and total saponins in Schisandra medicinal materials science research makes a tentative total saponin and schisandrin content limit with the result that each piece of content of total saponin in ginseng saponin Re (C48H82O18) shall not be less than9.4mg, containing Schisandra schizandrin (C24H32O7) meter shall not be less than0.63mg.The preliminarily stable results from the accelerated tests show the three batches of samples keep stable within6months of good performance, no change in appearance. The identification of TLC has a positive reaction and total saponins, schisandrin contents are qualified.