Purification And Hypoglycemic Effect In Vitro To Proanthocyanidin From Grape Seed Extracts

Xinjiang is China’s major viticultural areas, wine grape production accounted for1/4, so the production of waste-several thousands of tons per year to produce grapeyield enormous waste。In this study, grape seed as experimental material, purifiedwater enzyme complex extraction of grape seed proanthocyanidins, macroporousresin adsorption, Sephadex LH-20gel column chromatography grape seed extractproanthocyanidins be adopted in response surface analysis method for purificationconditions were optimized, high performance liquid chromatography analysis of thepurity of grape seed proanthocyanidins, and hypoglycemic activity of the purifiedproduct was evaluated in vitro.Enzyme complex by water extraction method of grape seed proanthocyanidins,on the basis of single factor experiment, the establishment of a mathematical modelusing quadratic response surface method and obtain the optimal conditions: theenzyme dosage0.8mg/g, pH5.5, hydrolysis temperature40℃, hydrolysis time4h,the leaching rate of71.38%theoretical verify the actual leaching tests showed thatunder these conditions was71.20%, with the theoretical value of the difference0.18,indicating that the quadratic reliable mathematical model.Static and dynamic adsorption tests showed macroporous resin HPD-400ofgrape seed proanthocyanidins have better adsorption capacity. After HPD-400macroporous resin adsorption HPD-400, by thin layer chromatography, HPLC todetermine the enrichment phase, each separate component and then purified bysephedaxLH-20gel column. sephedaxLH-20after gradient elution get fourcomponents. Component analysis by TLC, HPLC analysis, and infrared spectroscopyscans, identifies four components were purified with catechin, epicatechin,procyanidinB1, procyanidinB2. Grape seed extract, an extract component, macroporous resin separation andpurification components and sephedaxLH-20components, anti-vitro activity andnon-enzymatic glycation of proteins Inhibition of oxidation. The results showed that:different polar organic solvent extraction of grape seed extract, antioxidant extractcomponent and non-enzymatic glycation inhibition of ethyl acetate are the strongestpart; each component with the resulting macroporous resin separation in30%ethanolcomponent of the antioxidant activity and inhibition of non-enzymatic glycationseparated components were higher than the otherThe sephedaxLH-20purification of the resulting four monomers, with a totalreduction of capacity and non-enzymatic glycation inhibition strongest procyanidinB2; scavenging DPPH and OH-epicatechin is the strongest, followed by procyanidinB2monomer; after purification of each component in the antioxidant activity andinhibition of non-enzymatic glycation are higher than the crude extract from grapeseeds. Description After the separation and purification of the active ingredient ofgrape seed extract has been effectively enriched and purified monomer is the highestactivity of grape seed proanthocyanidins B2...