Expression And Clinical Significance Of CK2β In Gastric Carcinoma

Background and ObjectiveIn the worldwide, stomach carcinoma(gastric carcinoma) ranks fourth in theincidence of malignant tumors, and its mortality rate is at the second malignancies,particularly in Japan as the center of East Asia, a higher incidence of gastric cancerserious harm to human health. The high incidence of gastric countries belonging tothe northwest and eastern coastal areas of gastric cancer incidence was significantlyhigher than China’s southern region; persons died of stomach cancer in China eachyear about16million people, accounting for23%of all cancer deaths. Although ithas long been the development of mechanisms for its clinical diagnosis of gastriccancer, the treatment carried out extensive research, but so far, the cause of stomachcancer is still not very clear, yet a lot of baking deeper study. Is currently able todetermine, like other malignancies, like the development of gastric cancer is a resultof more than one kind of internal and external factors working together, but themechanism of its molecular biology, we know very little. Therefore, for the study ofmolecular mechanisms of gastric cancer, is a very important and has importantexperimental and clinical significance of the work.Protein kinase CK2was originally discovered in1954, once called caseinkinase Ⅱ. The protein kinase ubiquitous eukaryotic cells, and the messenger is a multifunctional non-dependent serine/threonine protein kinase, is composed oftwo catalytic subunits (CKα and CKα,) and two regulatory subunits (CK2β)composed of heterologous tetramer. Each subunit may be a free state, having asubstrate-level phosphorylation of protein kinase ability. Highly conserved proteinkinase CK2and versatile, which plays an important role in tumor development,invasion and metastasis. At present, studies show: gene and protein level β subunitof protein kinase CK2expression was significantly increased in including head andneck squamous cell cancer, colorectal cancer, endometrial cancer, renal cell cancerand other malignancies.20in the world since the1950s, has been quite a lot ofpeople began to study the protein kinase, where the relationship between the proteinkinase and on a variety of human tumors have also been studied and discussed.Research to date about the relationship between gastric cancer and CK2β few,therefore occur CK2β with gastric cancer, What is the relationship betweendevelopment, we still need to pay a lot of energy to explore and experiment. In thisstudy, by using immunohistochemistry (SABC method) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect CK2β gene in gastriccancer tissues and adjacent normal tissue levels in patients with gastric cancer inorder to understand CK2β in gastric cancer whether abnormal expression, and therelationship between CK2β expression and clinicopathological features of gastriccancer patients between the presence and explore its role in gastric carcinogenesis,development, invasion and metastasis surface for gastric cancer prevention,diagnosis and treatment provide new ideas.Material and method1．Study and specimen collectionAll patients underwent surgery; selected from the First Affiliated Hospital ofZhengzhou University of Gastrointestinal Surgery conducted from December2011to December2012period radical gastrectomy94patients with gastric cancerspecimens, all records are received D2or D3lymphadenectomy routine examination,and patients were not conducted during other treatments with chemotherapy,radiation therapy, integrative therapy prior to surgery, and postoperative pathological examination confirmed gastric cancer. All patients informed consent, approved bythe hospital ethics committee and reported. After all specimens collected within30min into the liquid nitrogen tank, and then placed in-80℃Thermol cryogenicrefrigerator to save. Access standards: pathological diagnosis of gastric cancermedical specimens; censored criteria: received chemotherapy prior to surgery,radiation therapy, combination therapy and patients treated with other biologicalspecimens. Gastric cancer staging reference AJCC (American Joint Committee onCancer) standard TNM staging. The patient’s gender, age, tumor location and TNMstage, extracted from the medical records of PACS system from the First AffiliatedHospital of Zhengzhou University.2．Using immunohistochemistry (SABC method) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect CK2β next94cases ofgastric carcinoma tissues and its protein expression and mRNA transcription level,and analyze the expression of CK2β correlation between clinical and pathologicalfeatures of patients with gastric cancer.3．All experimental results are used SPSS17.0software for data analysis.Cancer tissues, adjacent tissues using t-test, correlation analysis using correlationanalysis matching tables, and the relationship between clinicopathological factorswere χ2test, with P <0.05as statistically significant test.Results1．In CK2β94cases of gastric cancer expression in gastric carcinoma was55.3%(52/94); next to their corresponding gastric cancer tissues CK2β expressionrate is low, its expression was10.6%(10/94) and distribution of staining scatteredshallow and sparse. In CK2βmRNA gastric tissue was also higher than the level oftranscription next to their corresponding gastric cancer tissues (P <0.05).2．CK2β protein expression levels and CK2βmRNA transcript levels weregastric carcinoma patients with tumor stage, histological grade and other factorssignificantly associated (P <0.05)Conclusions1．CK2β expression levels in human gastric cancer tissues was significantly higher than its adjacent tissues, expression and clinicopathological features of gastriccancer CK2β are closely related.2．Gastric CK2β might be a potential therapeutic target molecule or marker.