The Relationship Between FAT10mRNA Expression And Feature Of Clinical Pathology In Gastric Carcinoma

BackgroundGastric cancer is one of the most common malignant tumor and a leading cause of the death world-wide. With the development and appliance of molecule biological technology, research in the mechanism of gastric cancer has probe into the level of gene. But, it hadn't identified the specific marker of molecule pathology in gastric carcinoma yet. For a tumor to become established, compromises in the control of tumor related gene are necessary. The Ubiquitin-like modifier (UBL) which has generated much interest in the scientific community is implicated to play important role in gastric cancer. The ubiquitin and ubiquitin-related families of proteins play instrumental roles in variouscellular processes including cell-cycle regulation as well ad cell death/apoptosis through modification of target proteins. Ubiquitin is a highly conserved 76 amino-acid polypeptide that plays an important role in the comjugation of target protein via the 26S proteosome pathway. The targeting of proteins for degradation by ubiquitin occurs through a cascade of events requiring participation of three enzymes, the ubiquitin-activating enzyme(E₁),the carrier protein(E₂) and the ubiquitin ligase(E₃). The role of ubiquitin -like family members are only beginning to be elucidated, two different families of ubiquitin-like proteins have been reported, the ubiquitin-domain proteins(UDP) and the ubiquitin-like modifiers(UBL). Several ubiquitin and ubiquitin-related proteins have been associated with cell-cycle-related processes and implicated in cancer.FAT10 is an 18KD protein comprising 165 amino-acid residues. It was originallydiscovered through the identification of expressed genes covering the HLA-F genomic locus. A potential role of FAT10 in amtigen presentation was suggested by its expression in mature B cells and dendritic cells, and by its ability to be generally and synergistically inducible with cytokines IFNr and TNFa. FAT10 belongs to the UBL family of proteins and contains two ubiquitin-like moieties fused in tandem. It is 29% identical to ubiquitin at its N-terminus and 36% identical at the C-terminus. It has the C-tenninal Gly-Gly residues and there is a comserved Lys residue in each moiety of FAT10 analogous to Lys48 of ubiquitin, which may serve as a potential site for polyubiquitination of FAT10. FAT10 has been reported to have potential involvement in cell-cycle regulation, and shown to bind noncovalently to the human spindle assembly checkpoint protein,MAD2, a protein responsible for maintaining spindle integrity suring mitosis. The inhibition of MAD2 functin has been associated with chromosomal instability, a characteristic of many cancers. Previous study was concerned mainly about the struction of the FAT10, the character of its expression, it's inducible factor and the function, there are no report about the relationship between the expression of FAT10 and the character of clinical pathology in gastric cancer.ObjectiveIn the present study, we examined the expression of FATlOmRNA in gastric cancer tissue, paracancerous tissue as well as their normal mucosa by RT-PCR. And analysed the relationship between the expression of FAT10 and the character of clinical pathology in gastric cancer.Materials and MethodsA total of 48 gastric cancer tissue, paracancerous tissue and their normal mucosa was obtained from tissue resected in surgury during Mar, 2003 and May,2004 in the 1 st affiliated hospital of Zhejiang university. 48 cases of gastric cancer contains 31 male,17 female, mean 57.7 years old, 32 had lymph-node metastasis,11 had distantmetastasis. In the present study, 48 cases of gastric cancer paracancerous tissue and their normal gastric mucosa was detected FAT10 mRNA expression using reverse transcription-polymerase chain reaction(RT-PCR). Observing the relation between FAT10 expression and sex, diameter of tumors, the position of tumor occurrence, gross type, the level of differentiation, Tumor depth, lymph nodes metastasis, distant metastasis, TNM staging.ResultsFATlOmRNA expressed in 37.5% gastric carcinomas(18 of 48...