Smad4and β-catenin Expression In Colon Polyps In Children

PurposeResearch Smad4and β-catenin in colon polyps in children’s expression andmechanism of action.MethodSelect the Second Affiliated Hospital of Zhengzhou University, Department ofPathology, July2005April2013surgical specimens children wax block intestinalpolyps,60cases were juvenile polyps. Which solitary juvenile polyps in36cases(19males,17females,12years of age in March, the average age of6years old±2.1years), multiple juvenile polyps in24patients (14males and10females, aged6-9months old, the average age of4±1.3years); take the wax block adult colonadenocarcinoma15cases (8males,7females, aged42-83years, mean56±12.1years), both are tubular glands cancer ⅱ level; normal mucosa specimens nextpolyps in10patients (5males and5females, aged5-13years, mean7±1.2years),were taken from the edge away from the polyp larger than normal mucosa of7cm.Immunohistochemical methods to measure the expression levels of selectedspecimens β-catenin and Smad4protein.Resultβ-catenin protein expression: normal group, β-catenin protein is mainly linearexpression, protein stained brown particles, mainly located in the cell membrane cell-cell junctions, the positive expression rate was100%; solitary juvenile polypsthe β-catenin protein expression was80.56%(29/36), a few specimens negativeexpression (7cases of plasma cells expressing specimens, two cases have a weakstaining cytoplasm and nucleus); multiple juvenile polyps β-catenin staining was66.67%(16/24)(8specimens cytoplasmic staining cytoplasm and nucleus of threecases of specimens stained); β-catenin in colorectal mucosa positive rate was26.7%(4/15), the expression of the cytoplasm and nucleus of the significant increase inthe expression of cell membranes have different degrees of reduction. Solitaryjuvenile polyps with multiple juvenile polyps β-catenin protein in the cytoplasm andnuclei were stained appearance. As a positive control adult poorly differentiatedadenocarcinoma of the colon, β-catenin protein ectopic expression. Afterquantitative immunohistochemical analysis, compared with the control group,β-catenin protein in solitary juvenile polyps, multiple juvenile polyps,adenocarcinoma increased in varying degrees, the difference was statistically Smad4protein expression: in the normal control group Smad4protein staining was stronglypositive, appears as brown, its protein was localized in the cytoplasm and the nucleusof expressing cells, showed diffuse or focal distribution, the positive expression rateof100%; solitary juvenile polyp tissue Smad4protein staining was positive, thepositive expression rate of88.89%(32/36); multiple juvenile polyps Smad4proteinstaining compared with the normal group and solitary juvenile polyps groupweakened, its positive rate was70.83%(17/24); Smad4colorectal adenocarcinomashowed a weak positive or no expression, the positive rate was46.67%(7/15).Solitary juvenile polyps group and adenocarcinoma stained positive rate by statisticalsoftware analysis, the difference was statistically significant; multiple juvenilepolyps group compared with adenocarcinoma stained positive rate, the differencewas not statistically significant. After quantitative immunohistochemical analysis,compared with the normal control group, Smad4protein expression were decreasedto varying degrees.ConclusionThe Children’s Institute selected samples of intestinal polyps, whether single ormultiple juvenile polyps juvenile polyps, which β-catenin protein expression compared with normal control group increased in varying degrees, and the change inthe expression of cellular localization, from the normal of the film into a linearexpression in the cytoplasm and nucleus have different degrees of staining sucker tipsoncogene plays an important role in the formation of intestinal polyps in children.The expression of Smad4protein in varying degrees to reduce, and it has a differentdegree of staining decreased, indicating that the tumor suppressor gene silly girl childin the role of intestinal polyps weakened. Both participated in the children’s intestinalpolyps, the development process..