Study On The Chemical And Biological Of Lonicerae Japonicae Flos

This dissertation is composed of two chapters. The first chapter showed the isolation and identification of compounds from Lonicerae japonicae Flos, and the antioxidant, anticoagulant as well as a-glucosidase inhibitory activity of different extracts were assayed. The second chapter summarized the study progress of chemical constituent and pharmaceutical of L. japonicae extracted by water.Chapter1. The chemical constituents and bioactivities of Lonicerae Japonicae Flos1. The chemical constituents from the L. japonicae were studied by column chromatography. Eight compounds were isolated from the L. japonicae and identified as pentacosylalcohol (1),β-sitosterol (2),5-hydroxy-7,4’-dimethoxyflavone (3), a-D-glucose (6), hexadecanoic acid (7).2. DPPH, ABTS, and FRAP assays were used to screen the antioxidant activity of L. japonicae. The study showed that ethyl acetate extract (DPPH IC50=25.23μg·mL-1, ABTS IC50=9.2μg·mL-1) had better DPPH and ABTS radical scavenging ability. Ethyl acetate extract (TEAC=2840.80±5.51μg·mL-1) for the reduction of Fe3+ability was stronger.3. The effect of extracts of L. japonicae on blood coagulation was investigated using Vitamin K1as coagulant control and breviscapine as anti-coagulant control. Water extract significantly prolonged the plasma recalcification time (P<0.001). Ethyl acetate extract and n-Butanol extract significantly reduced the plasma recalcification time (P<0.01).4. The inhibitory effect against a-glucosidase of different extracts from L. japonicae were screened in a96-well plate. Total extract (IC50=378.61μg·mL-1) and EtOAC extract (IC50=451.45μg·mL-1) showed stronger inhibitory effect against a-glucosidase, which were much higher than that of acarbose (IC50=1358.34μgm·L-1) as positive control.Chapter2. The study progress of the water extract from Lonicerae japonicae FlosThis chapter summarized the progress of chemical constituent and pharmaceutical of L. japonicae.