Effects Of PRL-3on Regulating Migration Ability Of Endometrial Cell With Endometriosis Patient And Impacts Of Estrogen And Progestin On Regulating The Migration Ability Induced By PRL-3

PurposeThis research studies the difference of morphological structure and migration ability of cells, which is on the basis of cultivating endometrial epithelium cells of normal people and patients with endometriosis by primary culture. By the method of treating cells by estrogen, progestin and Sodium Orthovanadate (SoV), the outcome measures the phosphatase of regenerating liver-3(PRL-3) expression and its difference, explores the effects of PRL-3on regulating the migration ability of endometrial epithelium cells, and investigate the pathogenesis of endometriosis which is easy to transfer and invade.Method1. Culture endometrial cells with endometriosis patient and normal patient., and compare morphological structure of the cells.2. Compare the migration ability of these different cells by scratch test.3. PRL-3gene expression were analysed by real-time PCR and PRL-3protein expression by western blot in these cells. 4. After treating estrogen, progestin and SoV,which is the inhibitor of PRL-3, confirm the alteration of cells’migration ability by scratch test measure the PRL-3expression and its variation, discuss the relationship between the PRL-3and difference of migration ability of cells.Result1. Endometrial epithelium consists of glandular epithelial cells and stromal cells. The glandular epithelial cells are positive with immunohistochemical staining of karatin, and stromal cells are positive with immunohistochemical staining of vimentin. With the cells subcultured, karatin positive cells decrease and vimentin positive cells have a higher proportion and become the dominant cell.2. The scratch test shows that after36hours, the migration distance of endometrial epithelium cells exceeds that of normal cells. The cells are divided into four groups. Control group was treated with2%standard FBS; SoV group was treated with0.5umol/l SoV+2%standard FBS; estrogen group was treated with10-6mol/l estrogen+2%standard FBS; progestin group was treated with10-5mol/l progestin+2%standard FBS; In Control group, migration distance of endometrial cells is greater than that in SoV group; In estrogen group, migration distance of endometrial cells is greater than that in Control group; In Control group, migration distance of endometrial cells is greater than that in progestin group; 3. The expression of PRL-3in endometrial cells of EMs patients is increased compared to that of normal people, for which the difference of those two expression has statistical means(P<0.05); the expression of PRL-3in endometrial cells cultured in control group is increased compared to that of cultured in SoV group, for which the difference of those two expression has statistical means(P<0.05); the expression of PRL-3in endometrial cells cultured in estrogen group is increased compared to that of cultured in control group, for which the difference of those two expression has statistical means(P<0.05); the expression of PRL-3in endometrial cells cultured in control group is Increased compared to that of cultured in progestin group, for which the difference of those two expression has statistical means(P<0.05).Conclusion1. The migration ability of endometrial epithelium cells of EMs patients is increased compared to that of the normal people. The PRL-3stimulates the cells migration ability. PRL-3can increase migration ability of endometrial cell in endometriosis through PRL-3signal.2. The intervention of estrogen is able to promot the migration of endometrial cell through increasing expression of PRL-3, while the progestin restrains the migration through decreasing expression of PRL-33. SOV can decrease migration ability by inhibit the expression of PRL-3.