Correlation Study Of MR Perfusion Imaging And AQP1in Rat Brain Glioma Peritumoral Edema Tissue

Objective:To explore the magnetic resonance perfusion weighted imaging(MR-DWI) features of peritumoral edema tissues in rat C6glioma, and the relationship between its aquaporin-1(AQP1) expression and regional cerebral blood volume (rCBV)Methods:C6glioma cells were injected stereotactically into the right caudate nucleus of35Wistar rats. about3～4weeks after implantation, all survival rats were examined by conventional MRI, PWI and enhanced T1WI scans. Postprocessing was done using the PWI specific software Function Tool,3equal-sized ROIs were drawn in peritumoral edema and the contralateral normal brain parenchyma to gain rCBV value. The rats were sacrificed immediately after MRIs cans, their whole brain were taken fixed to do peritumoral edema tissue continuous sections and the corresponding parts of HE and Immunohistochemical staining for AQP1. Pearson correlation analysis was used to determine the relations between rCBV value and the IOD value of AQP1expression.Results:About3～4weeks after implantation, the3equal-sized ROIs average rCBV value of glioma peritumoral edema tissue was (244.34±45.96). This was significantly higher than average rCBV value of the contralateral normal brain tissue (205.18±30.46). Two independent-sample t test of two groups showed statistically significant difference (P<0.05). The IOD value of AQP1expression of peritumoral edema tissues was (76595.35±1116.98).There was a positive correlation between rCBV value and the IOD value of AQP1expression (r=0.51,P<0.05).Conclusion:rCBV value of infiltrated peritumoral edema tissue in rat C6glioma was higher than contralateral brain tissue, and they can reflect the expression level of AQP1.