CaTiO₃Nanoparticle Distribution And Toxicity Effects Through Intraarticular Injection On SD Rats

Objective:To observe the CaTiO3nanoparticle distribution and the toxicity effects on SD rats through intraarticular injection.Methods:1.Detect the physical characteristics of CaTiO3nanoparticles, through scanning electron microscopy, transmission electron microscopy, X-ray diffraction and particle size analyzer.2.SD rats were divided into four groups, including low concentration group of0.2mg/ml, middle concentration group of2mg/ml, high concentration group of20mg/ml and saline control group.100ul liquids were injected into bilateral knee joint cavities. Take the injection once every other day and stop to observe closely after a total of four times of injection. Three months later, record the weight and collect blood samples to detect the serum indexes such as liver and kidney function. Collect and weight the specimens of bilateral knee joints, lung, heart, liver, spleen and kidney, then HE staining was taken to observe the tissue morphology.Results:1.The size of CaTiO3nanoparticle was about50nm, short rod or round, with high purity and the phenomenon of large particle agglomeration was not obvious.2.After the modeling, the rats’s activities reduced temporarily, without other abnormal behavior. Morphological changes and nanoparticle deposition were found in the internal organs, and detection of serum was abnormal, which indicated that the CaTiO3nanoparticles spread from the joint cavity to distant target organs.3. In the joint cavity, nanoparticle deposition were found in the synovial tissue and changed the synovial tissue morphology.Conclusion:In this experiment, we observed the potential toxicity effects of injection of CaTiO3nanoparticles in organs and knees of SD rats. The results of this experiment showed that wear particles stranded in the joint cavity had a potential impact on the physiological functions of the body. Patients after joint replacement surgery need regular check-ups with time going. Traditional nanometer coating on the plant need assess the effects of wear particles strictly.