| Objective We observed the reaction of the ovalbumin (OVA)-sensitized germ-free mice after inoculated by the feces of allergic and nonallergic SPF mice, to explore the effects of intestinal flora on the mechanismof food allergy.Methods The feces of allergic and non allergic SPF mice wastransferred separately to the germ-free mice, for allergic mice group (FA)and no response group (NR), each group of10. Inoculation after two weeks,two groups of7mice were set up food allergy model, called the experimentalgroup (FA-o group and NR-o); The other3for the control group (FA-group c and NR-group c).(1) The levels of OVA-sIgE in serum weremeasured by ELISA.(2) The tissues of small intestine were examined by HEstrain.(3) The engraftment and change of fecal flora were detected byDGGE.Results (1) After OVA sensitized, FA-o group sensitization rate was85.7%(6/7) in mice, and NR-o the sensitization rate is only14.3%(1/7) withserum OVA-sIgE levels higher than the control group X+2sd assensitization successful positive decision value;(2) HE staining showed thatFA-o group had more serious inflammation reaction than NR-o group, such as the Inflammatory cells infiltration, the focal necrosis and shedding of theepithelial cells in intestinal villi;(3) DGGE profiles and the correlationanalysis showed that after bacteria engraftment for2weeks, the overall florastructure of FA and NR group was obviously different; the DGGE bands oftwo groups were of marked difference; there was a very good separation oftwo groups in cluster analysis and principal component analysis (PCA);Sequencing results also showed that the Firmicutes in NR group werepredominate, whereas the Proteobacteria were advantage bacterium in FAgroup.Conclusions (1) Different microbes could affect the sensitivity of theinoculated mice on OVA, which suggested abnormal intestinal flora may bethe initiating factor in food allergies;(2) the sensitivity of a food allergycould be passed through the intestinal flora. |
PDF Full Text Request