The Role Of HBsAg In Regulating The Expression Of MiR-31in HepG2Cells

Objective: To investigate the effect of hepatitis B virus (HBV) andHepatitis B virus surface antigen (HBsAg) on microRNA-31(miR-31)expression in HepG2cells.Methods The stable HBsAg-overexpressing cell lines wereestablished by transfecting HepG2cells with an HBsAg-bearingmammalian expression vector and the clones were validated byEnzyme-Linked Immunosorbnent Assay(ELISA) andimmunohistochemistry. Sequencing detection for significant commommiRNAs, The expression level of one miRNAs, was measured by real-timepolymerase chain reaction (RT-PCR).Results: We have successfully constructed mammalian HBsAgexpression vector and generated the stable HBsAg-overexpressing HepG2cell line HepG2-H2. And find15commom miRNAs by Sequencingdetection,and miR-31selected for quantitative analysis. The expressionlevel of miR-31is higher in HepG2than that in HepG2.2.15cells, whichoverexpresses HBV (t=583.8,P<0.001). In addition, miR-31is up-regulatedin the stable HBsAg-overexpressing HepG2cell line(F=24.922,P<0.05). Conclusion: Intact hepatitis B virus represses miR-31expression inHepG2cells, and HBsAg overexpression leads to up-regulation of miR-31in hepatocarcinoma cells.