Effect Of Casticin On The EMT And Invasion In Human Cervical Hela Cells In Vitro

Objective To investigate the effects of casticin(CAS) inhibited the morphologicchanges of EMT and The migration and invasion induced by TGF-β1in HeLa cellline.Methods Induced epithelial-mesenchymal transition in Hela cells by differentconcentrations of TGF-β1. The morphological changes of EMT induced by TGF-β1in HeLa cells were observed under phase-contrast microscopy;The morphlogicalchanges of EMT induced by TGF-β1in HeLa cells caused by CAS in differentconcentrations were assesed by HE stain;Migration and invasion(with matrigel) assaywas conducted in the transwell chamber;The expressions of E-cadherin、N-cadherinand Twist were analyzed by Western blot.Results The HeLa cells were transformed by the polygonal epithelial cellsmorphology into the interstitial cells morphology after it were treated with TGF-β1at1.0、5.0and10.0ng/mL for72h, expressed as cells become spindle cells and theintercellular space was widened in concentration-dependent manner. the expression ofepithelial maker protein E-cadherin up-regulated and the mesenchymal maker proteinN-cadherin down-regulated after Hela cells were treated by TGF-β1at10.0ng/mL.CAS can inhibit the Hela cells in mesenchymal morphology (fusiformfibroblast-like) transformation after the cells were induced by TGF-β1at10.0ng/mL,and down-regulated expression of N-cadherin and up-regulated expressionof E-cadherin with the concentration-dependent manner. Compared with the controlgroup which was treated by TGF-β1at10.0ng/mL,the ability of migration andinvasion of HeLa cells was significantly reduced after the cells were treated by CASfor72h, while the expression of Twist down, in a concentration-dependent manner. Conclusion1. CAS can inhibit the effect of EMT induced by TGF-β1in HeLacell line.2.CAS can inhibit the ability of invasion and metastasis induced byTGF-β1in HeLa cell line.3.CAS can inhibit the mechanisms of EMT, invasion and metastasis ofinduced by TGF-β1in HeLa cell line may be related to reduced protein expressionassociated with Twist.