The Effect Of Fluvastatin Sodium To CHD Patient’s EPCs And HS-CRP

Objective: To explore the effect of different doses of fluvastatin sodium on thenumber of EPCs function and hs-CRP concentrations.Methods:74cases were recuited from September2012and March2013in the SouthChina University Second Affiliated Clinical College of Cardiology, the first admissionof patients, according to the World Health Organization (WHO) diagnostic criteria forselected coronary heart disease (CHD),of which48males and26females,46cases ofacute coronary syndrome (ACS), and28with stable angina pectoris (SAP).28patientswere randomly divided into two groups according to the order of admission:fluvastatin sodium40mg/day group,80mg/day group. Dates of patient age, gender,family history of coronary heart disease, hypertension medical history, drugs usedhistory and follow-up of eight weeks went collected.Peripheral blood mononuclearcells went gathered by density gradient centrifugation, which was inoculatedfibronectin-coated plates, cultured for7days under a fluorescence microscope toidentify FITC-UEA-I and acLDL-Dil.Double positive cells are considereddifferentiating EPCs; Flow cytometry was used to detect cell surface antigens CD34and VEGFR2determine the amount of EPCs; growth state MTT assay using cellproliferative activity was measured; enzyme-linked immunosorbent assay (ELISA) todetect the concentration of hs-CRP and compare the differences. Simultaneousdetection of all patients with total cholesterol (totaleholesterol, TC), low-densitylipoprotein cholesterol (low density lipoprotein Cholesterol, LDL-C), high densitylipoprotein cholesterol (high density lipoprotein Cholesterol, HDL one C) andtriglycerides (triglyeeride, TG) levels.Results:1.There were no significant differences between the two groups in age,gender,historyof hypertension and diabetes mellitus,smoking,weight,indexand family history at thebeginning of the study;There were also no significant differences between the twodrugs use during the follow-up,P﹥0.05； 2． Cells separated from peripheral blood using density gradient centrifugationmononuclear cells could induce growth factors in the VEGF and bFGF, differentiationof EPCs; identified by fluorescence microscopy FITC-UEA-I and acLDL-Dil.Doublestaining of EPCs cells were thought to be differentiating; flow cytometry as VEGFR2+and CD34+cells to the antibody-positive EPCs;3. ACS group compared with the control group significantly the number of EPCsincreased in ACS group.Compared with the control group but it proliferative capacitywas significantly impaired; significantly reduced the number of SAP group EPCscompared with the control group, impaired their proliferation; ACS Group the numberof patients with circulating EPCs, hs-CRP concentrations were significantly increased,significant positive correlation between the two;4. At the end of the study, serum TC, LDL-C and hs-CRP levels decreased comparedwith the start in two groups80mg/day group was more significant than40mg/daygroup; EPCs is outer periphery increased and its proliferative capacity enhanced, ofwhich80mg perday group was more significant than the40mg group.Conclusion:1Fluvastatin sodium in addition to lipid-lowering effect of statins there is ananti-inflammatory effect and increase the number of EPCs and the proliferation of cer-tain effect.2Fluvastatin sodium can lower serum TC, LDL-C and hs-CRP levels and increase thenumber and proliferation of EPCs,80mg perday play more significant role comparedwith40mg perday.