The Roles Of GnRH, SS And Their Receptors In Acute Terminal Ileitis By Craniocerebral Firearm Injury

Objective:We tested the concentration changes of gonadotropin-releasing hormone (GnRH), somatostatin(SS), and the expression levels of their receptors in acute terminal ileitis in experimental dogswith several types of stresses, to preliminarily study the internal link between stress hormonesand their receptores, revealing the association of stress with intestinal diseases.Methods:Healthy adult male mongrel dogs were randomlu divided into8groups by5in each group. Thegroups were as follows: Normal control group (A), normal temperature and humidity with highblood glucose group (G), high temperature and high humidity with high blood glucose group(B), high temperature and high humidity with normal blood glucose group (H), normaltemperature and humidity normal with glucose craniocerebral firearm injury group (C), normaltemperature and humidity with hyperglycemia craniocerebral firearm injury group (D), hightemperature and high humidity with normal glucose craniocerebral firearm injury group (E),high temperature and high humidity with hyperglycemia craniocerebral firearm injury group(F). C, D, E, F groups were models given pistol craniocerebral firearm wound.The study wasapproved by the local ethics committee, and all experiments were carried out health advicebased on experimental animal care and use of national institutions.After modeling, dogs were fixed supinely on the operation table, along with tracheal intubation,ECG monitoring, cardiopulmonary resuscitation and other rescue measures. Heart rate, bloodpressure and other vital signs were monitored after the injury; No other processings wereconducted but basic cardiac compression and artificial respiration extrusion airbag rescue.Abdominal operations were done immediately under sterile conditions, ileocecums were located by laparotomy, and1-3cm terminal ileum tissues were cut followed by immediatecentrifuge and preservation, then the stumps were anastomosed. According to the time gradient:0hour,0.5hour,1hour,1.5hours,2hours,5peripheral blood samples from each dog indifferent groups were collected, immediately centrifuged and preserved. In this process, wetried to extend dogs’ lives by intravenous transfusion. The concentration changes of GnRH andSS in peripheral blood were detected quantitatively by enzyme-lined immunosorbent assay(ELISA). The expression levels of GnRH receptor and SS receptor in terminal ileums in A, B,F group at each time point were detected by Western blot.Results：1. The concentration changes of GnRH in cavine plasma were as follows: at0hr, GnRHconcentrations in8experimental groups had no significant difference; at0.5hour, the GnRHlevels in peripheral blood from8groups were significantly reduced likely due to their ownregulation; with the extend of stress time, GnRH concentration increased in a time-dependentmanner, and obviously more than the initial concentration (0hour);2. The concentration changes of SS in cavine plasma were followed: at0hr, SSconcentrations in8experimental groups had no significant difference; at0.5hour, the SS levelsin peripheral blood from8groups were significantly reduced likely due to their own regulation;with the extend of stress time, SS concentration increased in a time-dependent manner, andobviously more than the initial concentration (0hour);3. The expression levels of GnRH receptor, SS receptor in terminal ileum tissues of A, B,F groups were detected by Western Blot. The results were followed: in acute stress, GnRHR,SSTR expression levels rose; the levels of GnRH receptor, SS receptor were positivelycorrelated with the concentration of GnRH, SS.Conclusions:1. Acute terminal ileitis by craniocerebral firearm injury increased GnRH concentration inplasma and the expression of its receptor; 2. Acute terminal ileitis by craniocerebral firearm injury increased SS concentration in plasmaand the expression of its receptor;3. GnRH, SS and their receptors are involved in the regulation of acute terminal ileitis bystress in a positive correlation in a certain time period.