| Objective: To investigate the changes and significance of goblet cells and itsmitochondria in the experimental acute terminal ileitis.Methods:40adult male experimental dogs randomly divided into normal controlgroup (group A), high temperature, high humidity and high blood sugar group (groupB), room temperature, normal humidity normal blood sugar craniocerebral firearminjury group (group C), room temperature normal humidity high blood sugarcraniocerebral firearm injury group (group D), high temperature and high humidity,normal blood sugar craniocerebral firearm injury group (group E), high temperatureand high humidity, high blood sugar craniocerebral firearm injury group (group F),room temperature normal humidity high blood sugar group (group G), hightemperature and high humidity normal blood sugar group (group H), there were5dogs in each group. Measure and record vital signs(Include heart rate, breathing rate)30minutes before the Experiment started. All the dogs were administered withlumianning (0.05mL·kg)-1，group A、B、G、H do not given other processing, Thedogs in group C, D, E, F were given craniocerebral firearm injury.2hours later,took all dogs from each group and collected their terminal ileum tissues which were5cm away from the ileocecal valves. We observed the tissues with naked eyes firstand then clice cut to observe them under microscope and electronic microscope. Toanalyze the changes and significance of goblet cells and its mitochondria in theexperimental acute terminal ileitis.Results1. The change of vital signs30minutes before the experiment, The vitalsigns(Include heart rate, respiratory rate)of every group were stable, there were nodifferences with statistical significance among each group (P>0.05).Two hours later,There were no obvious changes in the vital signs before and after the experiment in group A、G、C and D (P>0.05). The heart rate, breathing rate in group B、H、E andF were significantly higher then before (P <0.05), but there was not apparentlydifference between group B and H, group F and E (P>0.05).2. The pathologic changes of the terminal ileum tissue Intestinal mucosalepithelium, intestinal villi and microvilli were almost complete under the lightmicroscope and the electron microscope in group A and G, and a small amount ofinflammatory cell infiltration. In other groups, the intestinal mucosa had a differentdegree of damage. The pathological changes include mucosal villi disarranged evensplit or curled, lacteals, capillaries and lymphatic vessels extended, inflammatory cellinfiltration, red blood cell infiltrated or bleeding, degeneration and necrosis ofepithelial cells, and separated from epithelial layer. There were also edema,hemorrhage and cracking in muscularis mucosa. By chiu’s Intestinal mucosal lesion,there was no statistically significant difference between group G and group A (normalcontrol group)(P>0.05). The difference between group B、C、D、E、F、H and groupA had statistical significance (P <0.05), However, there was no statisticallysignificant difference between group H and group B、group C and group D、group Eand group F (P>0.05).3. The changes of the goblet cells①The change of number: The number of gobletcells in group A and G had no significant change, the number of goblet cells in othergroups were reduced in a different degree;②The change of morphological structure:The morphological structure of goblet cells in group A and G had no significantchange, the morphological structure of goblet cells in other groups had a differentdegrees of change, varying degrees of edema, secretion enhancements;③Thechange of its mitochondria: The number and morphological structure ofmitochondria in group A and G had no significant change, the number andmorphological structure of mitochondria in other groups had a different change, themitochondrion matrix became thinner, they swelled irregularly and their cristaefractured and become fuzzy. By Flameng’ stage division, there was no statisticallysignificant difference between group G and group A (normal control group)(P>0.05). The difference between group B, C, D, E, F, H and group A had statistical significance (P <0.05), However, there was no statistically significant differencebetween group H and group B (P>0.05)、group C and group D、group E and groupF (P>0.05).Conclusions1. Experimental acute terminal ileitis can make the intestinal villi and microvillidisarranged, split or curled, inflammatory cell infiltration, capillaries and lymphaticvessels extended etc.2. Experimental acute terminal ileitis can make the number of goblet cells onintestinal mucosa reduced, secretion enhance, and the mitochondria swelledirregularly and their cristae fractured and become fuzzy.3. The number of goblet cells reduced and their activity enhanced may be related tothe intestinal mucosal barrier was damaged caused by energy metabolism disorderafter intestinal epithelial cell mitochondria swelling, and to the enhancement ofcompensatory defense mechanism. |
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