Effect Of LAST1Over-expression On Proliferation And Cell Cycle Regulation Of A549Cells

OBJECTIVETo over-express large tumor suppressor gene1(LATS1), andinvestigate its effect on the proliferation and cell cycle regulation in lungcancer cell A549.METHODSLATS1gene coding region was cloned into lentivirus vector, and thenlentivirus particles were transfected into A549cells to up-regulate theexpression of LATS1gene. The expression of LATS1and YAP weredetected at both mRNA and protein levels by RT-PCR and Western blot.The apoptosis and cycle of cells were detected by flow cytometry. Theproliferation level of cells was detected by CCK-8.RESULTSThe expression levels of LATS1mRNA and protein wereup-regulated significantly after the lentivirus vector of LATS1overexpression transfected into A549cells,Meantime, the expression levels ofYAP mRNA and protein were down-regulated.5th days after transfection with lentivirus vector of LATS1over expression, the proliferation rate oftransfected cells was lower than untransfected cells (p<0.05). Flowcytometry showed that more cells of transfected went into phase G1andfewer cells went into phase S. Moreover, apoptosis rate was significantincreased in transfected cells (p<0.05).CONCLUSIONSLATS1can promote A549cells apoptosis, induce G1phase arrest andinhibit the cell proliferation by down-regulating the expression of YAP.